Demonstration of aberrant T-cell and natural killer-cell antigen expression in all cases of granular lymphocytic leukaemia
Demonstration of aberrant T-cell and natural killer-cell antigen expression in all cases of granular lymphocytic leukaemia
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DOI：
10.1046/j.1365-2141.2003.04201.x
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发表时间：
2003-03-01
影响因子：
6.5
通讯作者：
Hanson, CA
中科院分区：
文献类型：
--
作者：
Morice, WG;Kurtin, PJ;Hanson, CA
颗粒淋巴细胞白血病（GLL）的诊断需要免疫表型不同的t细胞（T-GLL）或自然杀伤细胞（NK-GLL）群体的存在。采用T和NK细胞相关抗原抗体对21例T- gll患者、11例NK- gll患者和20例正常对照进行流式细胞术免疫分型，以准确鉴别T- gll和NK- gll的区别特征。评估的NK抗原包括：CD16、CD57、CD94、CD161和杀伤抑制受体（KIRs） CD158a、CD158b和CD158e （p70）。所有T-GLL患者均存在异常的t抗原表达。CD57在T- gll中经常表达，然而，三分之一的患者显示部分CD57表达与正常对照者的T细胞相似。10例T-GLL为KIR阳性；都表达一个单一的KIR亚型。所有NK-GLL均表现出独特的异常免疫表型。4个NK-GLL表达一个单一的KIR亚型；其余7名患者缺乏所有检测的kir，这也是一个明显的异常发现。NK-GLL患者骨髓活检标本中CD8、TIA-1和颗粒酶B抗体的免疫过氧化物酶染色显示了先前在T-GLL中发现的疾病特异性独特染色模式。这些研究描述了诊断T-GLL的独特免疫表型特征，并提供了强有力的证据，证明NK-GLL与T-GLL一样，是一种克隆性淋巴细胞增生性疾病。
The diagnosis of granular lymphocytic leukaemia (GLL) requires the presence of an immunophenotypically distinct T-cell (T-GLL) or natural killer-cell (NK-GLL) population. Flow cytometric immunophenotyping was performed on 21 T-GLL patients, 11 NK-GLL patients and 20 normal control subjects using antibodies to T and NK cell-associated antigens in order to accurately identify the distinguishing features of T-GLL and NK-GLL. The NK antigens evaluated included: CD16, CD57, CD94, CD161, and the killing inhibitory receptors (KIRs) CD158a, CD158b and CD158e (p70). Abnormal T-antigen expression was present in all T-GLL patients. CD57 was frequently expressed in T-GLL, however, one-third of patients showed partial CD57 expression similar to that seen in T cells from normal control subjects. Ten T-GLL were KIR positive; all expressed a single KIR isoform. All NK-GLL showed a distinctive, abnormal immunophenotype. Four NK-GLL expressed a single KIR isoform; the remaining seven patients lacked all tested KIRs, which is also a distinct, abnormal finding. Immunoperoxidase staining of bone marrow biopsy specimens from NK-GLL patients with antibodies to CD8, TIA-1 and granzyme B revealed the disease-specific distinctive staining patterns previously found in T-GLL. These studies delineate the unique immunophenotypic features diagnostic of T-GLL and provide strong evidence that NK-GLL, like T-GLL, represents a clonal lymphoproliferative disorder.