Applications of electron-ion dissociation reactions for analysis of polycationic chitooligosaccharides in Fourier transform mass spectrometry
Applications of electron-ion dissociation reactions for analysis of polycationic chitooligosaccharides in Fourier transform mass spectrometry
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DOI：
10.1021/ac034477f
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发表时间：
2003-11-01
影响因子：
7.4
通讯作者：
Zubarev, RA
中科院分区：
文献类型：
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作者：
Budnik, BA;Haselmann, KF;Zubarev, RA
在FTICR质谱仪中通过电子-离子解离反应分析单质子化、双质子化和钠化五葡糖酰胺（GlcNAc）（5）、低聚葡糖胺（GlcN）（m）和（GlcN）（3）GlcN（30 H14：0），并与碰撞活化进行比较。发现一般裂解模式为具有全序列覆盖的不对称序列片段（B，和次要C，离子系列）。每个葡糖酰胺或葡糖胺残基的分子量信息可以容易地从离子系列获得。通过电子捕获解离的片段化揭示了与持续的非共振辐射碰撞激活解离（SORI-CAD）和电子诱导解离（EID）相比N-乙酰基部分的额外片段化。通过EID分析钠化GlcNAc（5）分子加合物离子，并与CAD进行比较。两种技术都提供了完整的序列覆盖。EID更有效，但CAD导致所有相关葡萄糖酰胺残基的跨环离子产物（0，2）A（n）和（2，4）A（n）。
Singly protonated, doubly protonated, and sodiated pentaglucosamide (GlcNAc)(5), oligoglucosamines (GlcN)(m), and (GlcN)(3)GlcN(30H14:0) were analyzed in an FTICR mass spectrometer by electron-ion dissociation reactions and compared to collision activation. The general fragmentation mode was found as the asymmetrical sequence fragments (B,, and minor C,, ion series) with full sequence coverage. Molecular mass information of each glucosamide or glucosamine residue can be readily obtained from the ion series. Fragmentation by electron capture dissociation revealed additional fragmentation of the N-acetyl moiety compared to sustained off-resonance irradiation collision-activated dissociation (SORI-CAD) and electron-induced dissociation (EID). Sodiated GlcNAc(5) molecular adduct ions were analyzed by EID and compared to CAD. Both techniques provided full sequence coverage. EID was more effective, but CAD resulted in the cross-ring ion products (0,2)A(n) and (2,4)A(n) for all relevant glucosamide residues.