Role of hydroxyl radical in modification of cell wall polysaccharides and aril breakdown during senescence of harvested longan fruit
Role of hydroxyl radical in modification of cell wall polysaccharides and aril breakdown during senescence of harvested longan fruit
复制标题
羟基自由基在龙眼果实衰老过程中细胞壁多糖修饰和假种皮分解中的作用
DOI：
10.1016/j.foodchem.2011.03.031
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发表时间：
2011-09-01
期刊：
影响因子：
8.8
通讯作者：
Jiang, Yueming
中科院分区：
文献类型：
--
作者：
Duan, Xuewu;Zhang, Haiyan;Jiang, Yueming
研究了活性氧（ROS），特别是羟基自由基（（中心点）OH）对体外和体内细胞壁分解以及龙眼果实假种皮分解的影响。 (中心点)OH在体外引起细胞壁物质(CWM)的分解，通过释放的总糖和糖醛酸增加以及CDTA可溶性果胶(CSP)和Na(2)CO(3)可溶性果胶(CO(3)-SP)的分子量下降来证明。此外，(中心点)OH导致CWM悬浮液中CO(3)-SP含量减少和CSP含量增加，表明CO(3)-SP转化为CSP。外源（中心点）OH 的应用加速了龙眼果实中的假种皮分解，而 t-半胱氨酸中心点 HCl（一种 ROS 清除剂）则延迟了该过程。此外，在（中心点）OH 处理的水果中观察到较低的 CWM 含量和果胶分子质量降低。这些结果表明，（中心点）OH 有助于细胞壁多糖的降解，导致龙眼果实中的假种皮分解。 (C) 2011 Elsevier Ltd. 保留所有权利。
The effects of reactive oxygen species (ROS), especially hydroxyl radical ((center dot)OH), on cellular wall disassembly in vitro and in vivo, and aril breakdown in longan fruit, were investigated. (center dot)OH caused the disassembly of cell wall material (CWM) in vitro, demonstrated by the increases of total sugars and uronic acid released, and the downshift in molecular mass of CDTA soluble pectin (CSP) and Na(2)CO(3) soluble pectin (CO(3)-SP). In addition, (center dot)OH resulted in decreased CO(3)-SP content and increased CSP content in CWM suspension, suggesting the conversion of CO(3)-SP to CSP. Application of exogenous (center dot)OH accelerated aril breakdown in longan fruit while the process was delayed by t-cysteine center dot HCl, a ROS scavenger. Furthermore, lower CWM content and decreased molecular mass of pectins were observed in (center dot)OH-treated fruit. These results indicated that (center dot)OH contributed to the degradation of cell wall polysaccharides, resulting in aril breakdown in longan fruit. (C) 2011 Elsevier Ltd. All rights reserved.