Proteomic analysis of seminal plasma in men with different spermatogenic impairment
Proteomic analysis of seminal plasma in men with different spermatogenic impairment
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DOI：
10.1111/j.1439-0272.2012.01275.x
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发表时间：
2012-08-01
期刊：
影响因子：
2.4
通讯作者：
Plaseska-Karanfilska, D.
中科院分区：
文献类型：
--
作者：
Davalieva, K.;Kiprijanovska, S.;Plaseska-Karanfilska, D.
精浆是许多男性生殖系统疾病（包括男性不育）生物标志物的潜在来源。精子发生正常和受损男性精浆中差异表达蛋白的鉴定和表征有助于阐明男性不育的分子基础。采用二维差分凝胶电泳（2d DIGE）技术，比较了正常精子组、弱精子组、少精子组和无精子组四组男性精浆蛋白表达谱。我们发现，与至少一个其他研究组相比，无精子症患者中有8种蛋白的表达有统计学意义的显著增加。差异表达点为纤维连接蛋白、前列腺酸性磷酸酶（PAP）、蛋白酶体亚基α -3型、β -2微球蛋白、半乳糖凝集素-3结合蛋白、催乳素诱导蛋白和细胞质非特异性二肽酶。值得注意的是，与所有其他组相比，无精子症患者的PAP增加。我们观察到正常精子、少精子和弱精子三组之间的蛋白表达没有统计学上的显著差异。我们认为，在我们的研究中鉴定的一组蛋白质，特别是PAP，有很大的潜力被用作无精子症的标志物。然而，需要进一步的研究来验证这些标志物在更大和独立的患者队列样本中的有效性，并阐明它们在男性不育症发病机制中的作用。
Seminal plasma is a potential source of biomarkers for many disorders of the male reproductive system including male infertility. The identification and characterisation of differentially expressed proteins in seminal plasma of man with normal and impaired spermatogenesis can help in the elucidation of the molecular basis of male infertility. We compared the protein expression profiles of seminal plasma from four different groups of men as follows: normozoospermic, asthenozoospermic, oligozoospermic and azoospermic groups, using two-dimensional differential gel electrophoresis (2-D DIGE). We found eight proteins with statistically significant increased expression in azoospermia compared with at least one of the other studied groups. The differentially expressed spots were fibronectin, prostatic acid phosphatase (PAP), proteasome subunit alpha type-3, beta-2-microglobulin, galectin-3-binding protein, prolactin-inducible protein and cytosolic nonspecific dipeptidase. Notably, PAP was increased in patients with azoospermia compared with that of all other groups. We have observed no statistically significant differences in protein expression between three of the groups: normozoospermic, oligozoospermic and asthenozoospermic. We suggest that the identified panel of proteins in our study especially PAP have a strong potential to be used as azoospermia markers. However, further investigations will be necessary to validate these markers in samples of larger and independent patient cohorts and to clarify their role in the pathogenesis of male infertility.