N-FORMYLMETHIONYL TRANSFER RIBONUCLEIC ACID IN MITOCHONDRIA FROM NEUROSPORA
N-FORMYLMETHIONYL TRANSFER RIBONUCLEIC ACID IN MITOCHONDRIA FROM NEUROSPORA
复制标题
DOI：
10.1021/bi00820a011
复制
发表时间：
1970-01-01
期刊：
影响因子：
2.9
通讯作者：
BARNETT, WE
中科院分区：
文献类型：
--
作者：
EPLER, JL;SHUGART, LR;BARNETT, WE
虽然细菌中蛋白质合成的起始机制似乎涉及fMet-tRNA，但对高等生物中的起始过程知之甚少。由于脉孢菌的线粒体含有一个蛋白质合成系统，其组分与细胞质的组分不同，因此我们检测了线粒体和细胞质提取物中的fMet-tRNA。来自线粒体的Met-tRNA可以用大肠杆菌提取物甲酰化，但相应的细胞质tRNA不能。这种线粒体fMet-tRNA是色谱分离的。
Although the mechanism for the initiation of protein synthesis in bacteriaappears to involve fMet-tRNA, little is known of the initiation process in higher organisms. Since the mitochondria of Neurospora contain a protein-synthesizing system with components distinct from those of the cytoplasm, we have examined bothmitochondrial and cytoplasmic extracts for fMet-tRNA. A Met-tRNA from the mitochondria can be formylated with an Escherichia coli extract, but the corresponding cytoplasmic tRN A cannot be. This mitochondrial fMet-tRNA is chromatographically separ-