Fat in Liver/Muscle Correlates More Strongly With Insulin Sensitivity in Rats Than Abdominal Fat
Fat in Liver/Muscle Correlates More Strongly With Insulin Sensitivity in Rats Than Abdominal Fat
复制标题
DOI：
10.1038/oby.2008.486
复制
发表时间：
2009-01-01
期刊：
影响因子：
6.9
通讯作者：
Lee, Hong K.
中科院分区：
文献类型：
--
作者：
Lim, Soo;Son, Kyu R.;Lee, Hong K.
肝内或肌内脂质（IHL/IML）含量已被报道与胰岛素抵抗相关。内脏脂肪也被证明与胰岛素抵抗有关。因此，我们研究了IHL/IML或内脏脂肪含量是否与胰岛素抵抗更密切相关。将20只Sprague-Dawley大鼠分为两组，一组给予普通饲料（RCD），另一组给予高脂饲料（HFD; 40%脂肪）。胰岛素敏感性指数（ISI）采用正葡萄糖钳夹法测定，内脏脂肪量采用计算机断层扫描（CT）测定，IHL/IML含量采用磁共振波谱（MRS）测定。测量体重、食物和水摄入量、体力活动、能量消耗、血脂、脂联素和高敏C反应蛋白（hsCRP）水平。在研究终点，HFD组的内脏脂肪和IHL/IML含量高于RCD组。IHL/IML含量与ISI的相关性高于内脏脂肪量。与内脏脂肪相比，脂联素和hsCRP水平与IML/IHL含量的相关性更强，尤其是在HFD组。在多元回归模型中，IHL/IML含量与ISI显著相关，而内脏脂肪含量与ISI无关。在IML/IHL对ISI的散点图中，RCD组和HFD组之间存在明显的区分，但内脏脂肪对ISI的散点图存在大量重叠。提示IHL/IML含量与胰岛素抵抗或动脉粥样硬化密切相关，是比内脏脂肪更好的胰岛素敏感性代谢指标。
Intrahepatic or intramuscular lipid (IHL/IML)content has been reported to be correlated with insulin resistance. Visceral fat has also been shown to be associated with insulin resistance. Thus, we investigated whether IHL/IML or visceral fat content is more closely associated with insulin resistance. Twenty Sprague-Dawley rats were divided into two groups based on regular chow diet (RCD) or high-fat diet (HFD; 40% fat). The insulin-sensitivity index (ISI) was determined by euglycemic glucose clamp study, the amount of visceral fat by computed tomography (CT), and the IHL/IML content by magnetic resonance spectroscopy (MRS). Weight, food, and water intake, physical activity, energy expenditure, lipid profile, adiponectin, and high-sensitivity C-reactive protein (hsCRP) levels were measured. At the study end point, visceral fat, and the IHL/IML content were higher in the HFD group than in the RCD group. The IHL/IML content was more highly correlated with ISI than was visceral fat amount. Stronger correlations were also found between adiponectin or hsCRP level and IML/IHL content than visceral fat, especially in the HFD group. Furthermore, the IHL/IML content was significantly associated with the ISI in the multiple regression models but visceral fat was not. There was clear discrimination between RCD and HFD groups in scatter plots of IML/IHL against the ISI, but substantial overlap in that of visceral fat against the ISI. This result suggests that IHL/IML contents are closely related with insulin resistance or atherosclerosis and is a better metabolic index of insulin sensitivity than the visceral fat.