Behavioral and molecular analyses suggest that circadian output is disrupted by disconnected mutants in D. melanogaster.
Behavioral and molecular analyses suggest that circadian output is disrupted by disconnected mutants in D. melanogaster.
复制标题
行为和分子分析表明，黑腹果蝇的昼夜节律输出被断开的突变体破坏。
DOI：
10.1002/j.1460-2075.1992.tb05020.x
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Rosbash,M
中科院分区：
文献类型：
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作者：
Hardin,PE;Hall,JC;Rosbash,M
断开（disco）基因的突变会破坏果蝇视觉系统中的神经细胞模式。这些突变也会影响成人的运动活动节奏，如迪斯科蝇在持续黑暗（DD）的条件下是不规律的。为了确定迪斯科突变体的昼夜节律起搏器状态，我们构建了pers双突变体（周期基因的短周期等位基因），并分析了它们在光照-黑暗周期（LD）条件下的行为节律，以及在光照-黑暗周期和光照-黑暗周期条件下周期基因表达的生化节律。结果表明，迪斯科蝇是有节奏感的，这表明它们有一个活跃的昼夜节律起搏器，可以被光所引导。他们还认为，迪斯科突变体阻断或干扰了位于中央起搏器与其输出之间的昼夜节律系统的元素，这些元素介导了明显的节奏。
Mutations in the disconnected (disco) gene act to disrupt neural cell patterning in the Drosophila visual system. These mutations also affect adult locomotor activity rhythms, as disco flies are arrhythmic under conditions of constant darkness (DD). To determine the state of the circadian pacemaker in disco mutants, we constructed with pers double mutants (a short period allele of the period gene) and assayed their behavioral rhythms in light‐dark cycles (LD), and their biochemical rhythms of period gene expression under both LD and DD conditions. The results demonstrate that disco flies are rhythmic, indicating that they have an active circadian pacemaker that can be entrained by light. They also suggest that disco mutants block or interfere with elements of the circadian system located between the central pacemaker and its outputs that mediate overt rhythms.