Moringa oleifera leaves alleviated inflammation through downregulation of IL-2, IL-6, and TNF-α in a colitis-associated colorectal cancer model
Moringa oleifera leaves alleviated inflammation through downregulation of IL-2, IL-6, and TNF-α in a colitis-associated colorectal cancer model
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DOI：
10.1016/j.foodres.2021.110318
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发表时间：
2021-04-06
影响因子：
8.1
通讯作者：
Loarca-Pina, G.
中科院分区：
文献类型：
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作者：
Cuellar-Nunez, M. L.;Gonzalez de Mejia, E.;Loarca-Pina, G.
由于全球结直肠癌发病率的上升，需要新的化学预防替代品。目的是评价辣木叶（MO）在结肠炎相关结肠癌发生模型中的化学预防活性。我们假设MO含有能够调节参与炎症反应和致癌作用的基因表达的生物活性化合物。将48只雄性小鼠（CD-1）分为6组：1：健康对照组; 2：氧化偶氮甲烷诱导的阳性对照组（AOM，10 mg/ Kg体重，腹膜内注射）和葡聚糖硫酸钠三个周期（DSS，饮用水中1.5%）;第3、4和5组用AOM/DSS诱导，并分别补充5%、10%和20%的MO;第6组：无病害诱导，添加20%的MO。将小鼠处理12周并安乐死。与AOM/DSS对照相比，辣木给药组在形态学和组织病理学参数方面存在显著差异（p < 0.05）。与AOM/DSS对照相比，在具有10%和20%MO的组中发现髓过氧化物酶活性（~ 50%）和脂质过氧化（1.9-3.1倍）降低（p < 0.05）。补充有10%MO的组显示粪便和盲肠内容物中丁酸盐和丙酸盐的显著增加（~ 3倍）。与AOM/DSS对照相比，补充有10%和20%MO的组显示血清中促炎细胞因子（MCP-1、IL-6、TNF-α）的减少。用10%MO处理诱导结肠组织中65个基因的差异表达，如IL 2、IL-6、TNF、IL-1 β和INF-γ。MO下调促炎介质，显示对炎症反应和结肠癌发生的化学预防特性。
New chemopreventive alternatives are needed due to the rising worldwide incidence of colorectal cancer. The objective was to evaluate the chemopreventive activity of Moringa oleifera leaves (MO) in a colitis-associated colon carcinogenesis model. We hypothesized that MO contain bioactive compounds capable of modulating the expression of genes involved in the inflammatory response and carcinogenesis. Forty-eight male mice (CD-1) were divided into six groups; 1: Healthy control; 2: Positive control induced with azoxymethane (AOM, 10 mg/ Kg body weight, intraperitoneal injection) and three cycles of dextran sodium sulfate (DSS, 1.5% in drinking water); groups 3, 4, and 5 were induced with AOM/DSS and supplemented with 5%, 10%, and 20% of MO, respectively; group 6: had no disease induction and supplemented with 20% of MO. Mice were treated for 12 weeks and euthanized. Significant differences (p < 0.05) were found for the moringa-administered groups in morphological and histopathological parameters compared to the AOM/DSS control. A decrease in myeloperoxidase activity (-50%) and lipid peroxidation (1.9-3.1 times) were found in groups with 10% and 20% of MO compared to the AOM/DSS control (p < 0.05). The group supplemented with 10% MO showed a significant increase (-3 times) in butyrate and propionate in fecal and cecal content. Groups supplemented with 10%, and 20% MO showed a reduction in proinflammatory cytokines in serum (MCP-1, IL-6, TNF-alpha) compared to the AOM/DSS control. Treatment with 10% MO induced differential expression of 65 genes in colon tissue such as IL2, IL-6, TNF, IL-1ss, and INF-gamma. MO downregulated proinflammatory mediators showing chemopreventive properties against inflammatory response and colon carcinogenesis.