ADMINISTRATION OF LACTOBACILLUS HELVETICUS NS8 IMPROVES BEHAVIORAL, COGNITIVE, AND BIOCHEMICAL ABERRATIONS CAUSED BY CHRONIC RESTRAINT STRESS
ADMINISTRATION OF LACTOBACILLUS HELVETICUS NS8 IMPROVES BEHAVIORAL, COGNITIVE, AND BIOCHEMICAL ABERRATIONS CAUSED BY CHRONIC RESTRAINT STRESS
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DOI：
10.1016/j.neuroscience.2015.09.033
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发表时间：
2015-12-03
期刊：
影响因子：
3.3
通讯作者：
Jin, F.
中科院分区：
文献类型：
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作者：
Liang, S.;Wang, T.;Jin, F.
越来越多的研究表明，肠道微生物群参与了应激相关疾病的病理生理学。慢性压力可导致行为、认知、生化和肠道微生物群畸变。肠道细菌可以通过微生物群-肠道-脑轴（主要包括免疫、神经内分泌和神经通路）与宿主沟通，影响大脑和行为。据推测，益生菌的管理可以改善慢性应激引起的抑郁症。为了验证这一假说，本研究建立了慢性束缚应激抑郁模型。成年无特定病原体（SPF）Sprague-Dawley大鼠接受21天的束缚应激，然后进行行为测试（包括蔗糖偏好测试（SPT）、高架迷宫测试、旷场测试（OFT）、物体识别测试（ORT））物体放置测试（OPT））和生化分析。在应激期间每天补充瑞士乳杆菌NS 8直到实验结束，选择性5-羟色胺再摄取抑制剂（SSRI）西酞普兰（CIT）作为阳性对照。结果表明，L. helveticus NS 8改善慢性束缚应激诱导的行为（焦虑和抑郁）和认知功能障碍，显示出与CIT相似且优于CIT的效果。L.与慢性应激大鼠相比，瑞士仓鼠NS 8组大鼠血浆皮质酮（CORT）和促肾上腺皮质激素（ACTH）水平降低，血浆白细胞介素-10（IL-10）水平升高，海马5-羟色胺（5-HT）和去甲肾上腺素（NE）水平恢复，海马脑源性神经营养因子（BDNF）mRNA表达增加。综上所述，这些结果表明L.研究表明，慢性束缚应激抑郁大鼠中的瑞士小球藻NS 8，这种作用可能是由于微生物群-肠-脑轴。同时也提示了L.瑞士人NS 8在压力相关的抑郁症和其他类型的抑郁症中的作用。(C)2015年IBRO。由爱思唯尔有限公司出版。保留所有权利。
Increasing numbers of studies have suggested that the gut microbiota is involved in the pathophysiology of stress-related disorders. Chronic stress can cause behavioral, cognitive, biochemical, and gut microbiota aberrations. Gut bacteria can communicate with the host through the microbiota-gut-brain axis (which mainly includes the immune, neuroendocrine, and neural pathways) to influence brain and behavior. It is hypothesized that administration of probiotics can improve chronic-stress-induced depression. In order to examine this hypothesis, the chronic restraint stress depression model was established in this study. Adult specific pathogen free (SPF) Sprague-Dawley rats were subjected to 21 days of restraint stress followed by behavioral testing (including the sucrose preference test (SPT), elevated-plus maze test, open-field test (OFT), object recognition test (ORT), and object placement test (OPT)) and biochemical analysis. Supplemental Lactobacillus helveticus NS8 was provided every day during stress until the end of experiment, and selective serotonin reuptake inhibitor (SSRI) citalopram (CIT) served as a positive control. Results showed that L. helveticus NS8 improved chronic restraint stress-induced behavioral (anxiety and depression) and cognitive dysfunction, showing an effect similar to and better than that of CIT. L. helveticus NS8 also resulted in lower plasma corticosterone (CORT) and adrenocorticotropic hormone (ACTH) levels, higher plasma interleukin-10 (IL-10) levels, restored hippocampal serotonin (5-HT) and norepinephrine (NE) levels, and more hippocampal brain-derived neurotrophic factor (BDNF) mRNA expression than in chronic stress rats. Taken together, these results indicate an anti-depressant effect of L. helveticus NS8 in rats subjected to chronic restraint stress depression and that this effect could be due to the microbiota-gut-brain axis. They also suggest the therapeutic potential of L. helveticus NS8 in stress-related and possibly other kinds of depression. (C) 2015 IBRO. Published by Elsevier Ltd. All rights reserved.