Colony Formation by Human Haemopoietic Precursor Cells Cultured in Semi‐solid Agar in Diffusion Chambers
Colony Formation by Human Haemopoietic Precursor Cells Cultured in Semi‐solid Agar in Diffusion Chambers
复制标题
扩散室半固体琼脂中培养的人类造血前体细胞的集落形成
DOI：
10.1111/j.1365-2141.1975.tb00837.x
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发表时间：
1975
影响因子：
6.5
通讯作者：
I. Douglas
中科院分区：
文献类型：
--
作者：
M. Gordon;N. Blackett;I. Douglas
描述了一种允许血液学正常人骨髓细胞集落生长的技术。细胞由植入辐照小鼠腹腔的改良Millipore扩散室内的半固体琼脂培养基支持。孵育9天后，在这些琼脂扩散室中发现含有多达1000个细胞的菌落。到目前为止，研究的所有血液学正常患者均产生菌落，大多数在每2 × 105个接种的骨髓细胞中有10至40个菌落。
A technique which allows colony growth of haematologically normal human bone marrow cells is described. The cells are supported by semi‐solid agar‐medium inside modified Millipore diffusion chambers implanted in the peritoneal cavity of irradiated mice. After 9 days incubation colonies containing up to 1000 cells are found in these Agar Diffusion Chambers. All haematologically normal patients studied so far produced colonies, the majority with between 10 and 40 colonies per 2 × 105 bone marrow cells inoculated.