Rice receptor for chitin oligosaccharide elicitor does not couple to heterotrimeric G-protein:: Elicitor responses of suspension cultured rice cells from Daikoku dwarf (d1) mutants lacking a functional G-protein α-subunit
Rice receptor for chitin oligosaccharide elicitor does not couple to heterotrimeric G-protein:: Elicitor responses of suspension cultured rice cells from Daikoku dwarf (d1) mutants lacking a functional G-protein α-subunit
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DOI：
10.1034/j.1399-3054.2002.1160313.x
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发表时间：
2002-11-01
影响因子：
6.4
通讯作者：
Shibuya, N
中科院分区：
文献类型：
--
作者：
Tsukada, K;Ishizaka, M;Shibuya, N
我们发现水稻N -乙酰壳寡糖激发子受体与异三聚体g蛋白（g蛋白）不偶联，而g蛋白是许多生物体各种细胞反应中从细胞表面到下游效应器的最重要的信号转导器之一。利用缺乏功能性g蛋白α亚基的突变水稻细胞，将悬浮培养的含有g蛋白α亚基编码区突变的Daikoku矮化（d1）突变水稻细胞（Fujisawa et al. 1999）与相应亲本细胞系对N -乙酰壳寡糖（寡甲壳素）激发子的细胞反应进行了比较。诱导剂诱导的所有细胞反应，如培养基碱化、活性氧的产生、早期反应基因、PAL和PR基因的表达以及植物抗毒素的产生，在突变型和野生型细胞系中基本相同。考虑到在包括水稻在内的许多植物物种中存在g蛋白α -亚基的单拷贝基因，这些结果有力地支持了上述结论。
We present evidence that the rice receptor for N -acetylchitooligosaccharide elicitor does not couple to heterotrimeric G-protein (G-protein), one of the most important signal transducers from the cell surface to down-stream effectors in various cellular responses of many organisms. Using mutant rice cells lacking functional G-protein alpha-subunit, cellular responses of suspension-cultured rice cells derived from Daikoku dwarf (d1 ) mutants that were shown to contain mutations in the coding region of the G-protein alpha-subunit (Fujisawa et al. 1999) to N -acetylchitooligosaccharide (oligochitin) elicitor were compared with those of the corresponding parent cell lines. All the elicitor-induced cellular responses, such as medium alkalinization, generation of reactive oxygen species, expression of early responsive genes, PAL and PR genes, and production of phytoalexin, were basically identical in the mutant and wild type cell lines. Considering the reported presence of a single copy gene for the G-protein alpha-subunit in many plant species including rice, these results strongly support the above conclusion.