INVIVO EFFECTS OF ESCHERICHIA-COLI ENDOTOXEMIA ON DIAPHRAGMATIC MICROCIRCULATION IN RATS
INVIVO EFFECTS OF ESCHERICHIA-COLI ENDOTOXEMIA ON DIAPHRAGMATIC MICROCIRCULATION IN RATS
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DOI：
10.1152/jappl.1992.72.6.2219
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发表时间：
1992-06-01
影响因子：
3.3
通讯作者：
AUBIER, M
中科院分区：
文献类型：
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作者：
BOCZKOWSKI, J;VICAUT, E;AUBIER, M
采用活体视频显微镜观察大肠杆菌内毒素对大鼠膈微循环的影响。将大鼠分为3组：1)静脉注射10 mg/kg大肠杆菌内毒素（E组，n = 25）， 2)静脉注射0.9%无菌NaCl (C组，n = 20), 3)动脉导管缩小血管容量诱导出血（H组，n = 15）。每隔15分钟测量一次平均血压（BP）和小动脉直径，每隔30分钟测量1小时毛细血管灌注模式。E组和h组血压下降相似，而c组血压保持不变。根据小动脉在神经网络中的相对位置，将其分为第二（A2, n = 46）、第三（A3, n - 22）和第四（A4, n = 21）级。三组的基底直径相同：C组为38.16、17.33、6.80 mu-m；E组38.17、17.41、7.04-mu-m；H组A2、A3、A4分别为37.82、19.19、6.99 μ m。观察期间，E组和H组A2小动脉血管收缩相似，但c组无明显收缩。而三组A3、A4小动脉直径均无明显变化。E组毛细血管灌注明显受损：60 min时，E组未灌注毛细血管比例为40.92 +/- 6.65%，C组为21.17 +/- 5.45% (P < 0.05)， C组为18.18 +/- 8%。H组为11% （P < 0.05）。我们得出结论，大肠杆菌内毒素血症对膈微循环产生显著的体内影响，其特征是1)A2膈小动脉收缩，这可能与动脉低血压有关；2)膈毛细血管灌注受损。
We investigated the effects of Escherichia coli endotoxin administration on diaphragmatic microcirculation in rats by in vivo videomicroscopy. Rats were allocated into three groups: 1) intravenous inoculation of 10 mg/kg of E. coli endotoxin (group E, n = 25), 2) intravenous inoculation of sterile 0.9% NaCl (group C, n = 20), and 3) induction of a controlled hemorrhage by reducing the vascular volume via an arterial catheter (group H, n 15). Mean blood pressure (BP) and arteriolar diameters were measured at 15-min intervals and capillary perfusion pattern at 30-min intervals for 1 h. BP decreased similarly in groups E and H, whereas it was maintained in group C. Arterioles were classified as second (A2, n = 46), third (A3, n - 22), and fourth (A4, n = 21) order, according to their relative localization in the network. Basal diameters were the same in the three groups: 38.16, 17.33, and 6.80-mu-m in group C; 38.17, 17.41, and 7.04-mu-m in group E; and 37.82,19.19, and 6.99-mu-m in group H for A2, A3, and A4, respectively. During the observation period, a significant and similar vasoconstriction of A2 arterioles was observed in groups E and H but not in group C. By contrast, in the three groups, no significant changes in diameter were found for the A3 and A4 arterioles. Capillary perfusion was markedly impaired in group E: at 60 min the percentage of non-perfused capillaries was 40.92 +/- 6.65% in group E compared with 21.17 +/- 5.45% in group C (P < 0.05) and 18.18 +/- 8. 11% in group H (P < 0.05). We conclude that E. coli endotoxemia produces a marked in vivo effect on diaphragmatic microcirculation that is characterized by 1) a constriction of A2 diaphragmatic arterioles, which could be related to arterial hypotension, and 2) an impaired diaphragmatic capillary perfusion.