Prostaglandin F 2 a upregulates Slit/Robo expression in mouse corpus luteum during luteolysis
Prostaglandin F 2 a upregulates Slit/Robo expression in mouse corpus luteum during luteolysis
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发表时间：
2013
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通讯作者：
Xuejing Zhang;Jianhua Li;Jiali Liu;Hao-shu Luo;K. Gou;S. Cui;Bioscientifica Ltd
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作者：
Xuejing Zhang;Jianhua Li;Jiali Liu;Hao-shu Luo;K. Gou;S. Cui;Bioscientifica Ltd
前列腺素F2 a（PGF 2a）是触发黄体（CL）退化的关键因素.此外，据报道，Slit/Robo信号转导参与调节黄体溶解。然而，PGF 2 a和Slit/Robo在黄体溶解过程中的相互作用仍有待证实.本研究旨在确定小鼠CL中黄体溶解是否受PGF 2 a和Slit/Robo相互作用的调节。实时荧光定量PCR和免疫组化结果显示，Slit 2及其受体Robo 1在小鼠CL中高度特异性共表达。功能研究表明，Slit/Robo通过促进细胞凋亡和上调caspase 3表达参与小鼠黄体溶解。体内外研究均表明，PGF 2 a通过蛋白激酶C依赖的ERK 1/2和P38 MAPK信号通路显著增加黄体溶解过程中Slit 2和Robo 1的表达，而Slit/Robo信号通路抑制剂显著降低PGF 2 a对黄体溶解的刺激作用。这些发现表明Slit/Robo信号通过介导CL中的PGF 2 a信号通路在PGF 2 a诱导的黄体溶解中起重要作用。
Prostaglandin F 2 a (PGF 2 a ) is a key factor in the triggering of the regression of the corpus luteum (CL). Furthermore, it has been reported that Slit/Robo signaling is involved in the regulation of luteolysis. However, the interactions between PGF 2 a and Slit/Robo in the progression of luteolysis remain to be established. This study was designed to determine whether luteolysis is regulated by the interactions of PGF 2 a and Slit/Robo in the mouse CL. Real-time PCR and immunohistochemistry results showed that Slit2 and its receptor Robo1 are highly and specifically co-expressed in the mouse CL. Functional studies showed that Slit/Robo participates in mouse luteolysis by enhancing cell apoptosis and upregulating caspase3 expression. Both in vitro and in vivo studies showed that PGF 2 a significantly increases the expression of Slit2 and Robo1 during luteolysis through protein kinase C-dependent ERK1/2 and P38 MAPK signaling pathways, whereas an inhibitor of Slit/Robo signaling significantly decreases the stimulating effect of PGF 2 a on luteolysis. These findings indicate that Slit/Robo signaling plays important roles in PGF 2 a -induced luteolysis by mediating the PGF 2 a signaling pathway in the CL.