Daily spawning and development of sensitivity to gonadotropin and maturation-inducing steroid in the oocytes of the bambooleaf wrasse, Pseudolabrus japonicus
Daily spawning and development of sensitivity to gonadotropin and maturation-inducing steroid in the oocytes of the bambooleaf wrasse, Pseudolabrus japonicus
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竹叶濑鱼卵母细胞的日常产卵和对促性腺激素和成熟诱导类固醇敏感性的发育
DOI：
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发表时间：
2004
影响因子：
1.4
通讯作者：
M. Kashiwagi
中科院分区：
文献类型：
--
作者：
M. Matsuyama;S. Morita;T. Nasu;M. Kashiwagi
研究了竹濑鱼卵母细胞的发育周期，以阐明海洋硬骨鱼卵母细胞成熟的内分泌机制。一只雌鱼和两只雄鱼一起饲养，在17天的圈养中每天产卵，这表明该物种是每天产卵的。卵巢组织学显示，最大卵母细胞的生发囊泡迁移在12:00 - 3:00进行，生发囊泡破坏（GVBD）在6:00 - 9:00完成，排卵和产卵发生在6:00 - 9:00。人绒毛膜促性腺激素（HCG）和17α，20β-二羟基-4-妊娠-3- 1 （17,20β- p）是最有效的GVBD甾体诱导剂之一。在诱导最终卵母细胞成熟的过程中，在一天中的八个不同时间进行了检查。卵母细胞对HCG和类固醇的反应在一天中的不同时间有所不同。9:00 h HCG可诱导GVBD，而17,20β-P不能诱导GVBD。12:00 ~ 18:00 h HCG和17,20β-P均可诱导GVBD。在没有任何激素治疗的情况下，GVBD和排卵均在0:00至6:00之间自发发生。这些结果清楚地表明，竹濑鱼卵母细胞具有日成熟周期。卵母细胞对HCG的反应早于对17,20β- p的反应。提示促性腺激素（GTH）诱导了对17,20 β-P的敏感性。
The cycle of oocyte development of the bambooleaf wrasse, Pseudolabrus japonicus, was studied to elucidate the endocrinological mechanism of oocyte maturation in a marine teleost. A single female reared with two males spawned every day for 17 days in captivity, indicating that this species is a daily spawner. Ovarian histology revealed that germinal vesicle migration of the largest oocytes progressed from 12:00 to 3:00 h, and germinal vesicle breakdown (GVBD) was completed at 6:00 h. Ovulation and spawning occurred between 6:00 and 9:00 h. The effectiveness of human chorionic gonadotropin (HCG) and 17α,20β-dihydroxy-4-pregnen-3-one (17,20β-P), which is one of the most potent steroidal inducers of GVBD in bambooleaf wrasse oocytes, in inducing final oocyte maturation was examined at eight different times of the day. The responsiveness of the oocyte to HCG and steroid differed at different times of the day. The GVBD could be induced by HCG but not 17,20β-P at 9:00 h. Between 12:00 and 18:00 h, not only HCG but also 17,20β-P induced GVBD. Both GVBD and ovulation spontaneously occurred between 0:00 and 6:00 h without any hormonal treatment. These results clearly showed that the oocyte of the bambooleaf wrasse possessed a diurnal maturation cycle. Responsiveness of oocytes to HCG appeared earlier than responsiveness to 17,20β-P. This suggests that sensitivity to 17,20 β-P is induced by gonadotropic hormone (GTH).
影响因子：
3.6
作者：
PATINO, R;THOMAS, P
通讯作者：
THOMAS, P