Structural alterations in a component of cytochrome c oxidase and molecular evolution of pathogenic Neisseria in humans.
Structural alterations in a component of cytochrome c oxidase and molecular evolution of pathogenic Neisseria in humans.
复制标题
DOI：
10.1371/journal.ppat.1001055
复制
发表时间：
2010-08-19
期刊：
影响因子：
6.7
通讯作者：
Koomey M
中科院分区：
文献类型：
--
作者：
Aspholm M;Aas FE;Harrison OB;Quinn D;Vik A;Viburiene R;Tønjum T;Moir J;Maiden MC;Koomey M
奈瑟氏菌属内的三种密切相关的细菌物种对人类疾病和健康具有重要意义。脑膜炎奈瑟氏菌是脑膜炎的主要原因，而淋病奈瑟氏菌是性传播疾病淋病的病原体，而内酰胺奈瑟氏菌是儿童常见的无害病原体。比较基因组学还没有产生明确的见解，哪些因素决定了独特的宿主-寄生虫的关系表现出的每一个，因为作为一个群体，他们显示出显着的保守水平的核苷酸序列，基因内容和共线性。在这里，我们发现了两个罕见的基因编码的细胞色素cbb 3氧化酶的CcoP蛋白质组分的变化，这是遗传信息。一个是单核苷酸多态性导致CcoP截短，作为物种N的分子标记。脑膜炎我们继续表明，祖先ccoP基因是由独特的基因复制和融合事件产生的，并且特异性且完全分布在奈瑟氏球菌属的物种中。令人惊讶的是，我们发现，工程菌株表达的两种CcoP形式有条件地不同的能力，以支持亚硝酸盐依赖性，微需氧生长介导的NirK，亚硝酸盐还原酶。因此，我们建议，在CcoP结构域的结构和随后的功能改变的变化是连续的，适应性辐射在这些集合种群的关键特征。这些发现提供了一个戏剧性的例子，说明核心代谢蛋白的罕见变化如何与重大的宏观进化转变相关联。他们还展示了分子水平的进化变化如何与代谢创新及其逆转联系起来，并展示了如何利用基因型来推断单个宿主内适应度景观的改变。近缘种N. meningitidis、脑膜炎奈瑟氏球菌（N.淋病奈瑟菌和淋病奈瑟菌。在人体内，内酰胺只在粘膜表面定植。而N.淋病导致淋病，其他两个物种主要在没有疾病的情况下在宿主中持续存在。N.然而，脑膜炎偶尔也会引起严重的危及生命的疾病。人们对决定每个物种所表现出的独特的人类相互作用的因素和元素知之甚少。此外，这些物种之间的进化关系也很难描述。在这里，我们描述了两个连续的改变，在一个单一的基因，可以连接到所有物种内的奈瑟氏球菌属，然后物种N。脑膜炎我们还表明，这些签名的变化有表型的影响核心呼吸代谢过程的后果。这些发现对单一宿主内相关细菌物种的进化具有重要意义，并为创新适应的偶发性和可逆性提供了新的视角。
Three closely related bacterial species within the genus Neisseria are of importance to human disease and health. Neisseria meningitidis is a major cause of meningitis, while Neisseria gonorrhoeae is the agent of the sexually transmitted disease gonorrhea and Neisseria lactamica is a common, harmless commensal of children. Comparative genomics have yet to yield clear insights into which factors dictate the unique host-parasite relationships exhibited by each since, as a group, they display remarkable conservation at the levels of nucleotide sequence, gene content and synteny. Here, we discovered two rare alterations in the gene encoding the CcoP protein component of cytochrome cbb 3 oxidase that are phylogenetically informative. One is a single nucleotide polymorphism resulting in CcoP truncation that acts as a molecular signature for the species N. meningitidis. We go on to show that the ancestral ccoP gene arose by a unique gene duplication and fusion event and is specifically and completely distributed within species of the genus Neisseria. Surprisingly, we found that strains engineered to express either of the two CcoP forms conditionally differed in their capacity to support nitrite-dependent, microaerobic growth mediated by NirK, a nitrite reductase. Thus, we propose that changes in CcoP domain architecture and ensuing alterations in function are key traits in successive, adaptive radiations within these metapopulations. These findings provide a dramatic example of how rare changes in core metabolic proteins can be connected to significant macroevolutionary shifts. They also show how evolutionary change at the molecular level can be linked to metabolic innovation and its reversal as well as demonstrating how genotype can be used to infer alterations of the fitness landscape within a single host. The closely related bacterial species N. meningitidis, N. gonorrhoeae and N. lactamica exclusively colonise mucosal surfaces in humans. While N. gonorrhoeae leads to gonorrhea, the other two species persist mainly in their host in the absence of disease. N. meningitidis does occasionally cause severe, life threatening illness, however. Little is known about the factors and elements that dictate the unique human interactions exhibited by each species. Moreover, the evolutionary relationships between these species are poorly characterized. Here, we describe two successive alterations in a single gene that can be linked first to all species within the genus Neisseria and then the species N. meningitidis. We also show these signature alterations have phenotypic consequences by affecting core respiratory metabolic processes. These findings have significant implications for the evolution of related bacterial species within a single host and provide a novel perspective on the episodic and reversible nature of innovative adaptation.
登录
查看更多内容
影响因子：
3.2
作者：
Heurlier, Karin;Thomson, Melanie J.;Moir, James W. B.
通讯作者：
Moir, James W. B.
影响因子：
5.6
作者：
Boulanger, MJ;Murphy, MEP
通讯作者：
Murphy, MEP
影响因子：
3.3
作者：
Didelot, Xavier;Falush, Daniel
通讯作者：
Falush, Daniel
影响因子：
3.9
作者：
Deeudom, M;Rock, J;Moir, J
通讯作者：
Moir, J
影响因子：
3
作者：
Ellis, Mark J.;Grossmann, J. Guenter;Hasnain, S. Samar
通讯作者：
Hasnain, S. Samar