Involvement of aquaporin-5 in differentiation of human gastric cancer cells
Involvement of aquaporin-5 in differentiation of human gastric cancer cells
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DOI：
10.1007/s12576-008-0017-3
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发表时间：
2009-03-01
影响因子：
2.3
通讯作者：
Sakai, Hideki
中科院分区：
文献类型：
--
作者：
Watanabe, Tomoko;Fujii, Takuto;Sakai, Hideki
关于水通道蛋白（AQP）水通道在人类胃癌中的功能知之甚少。在人胃的上部或中部，我们发现肠型腺癌中AQP5蛋白的表达水平显着高于伴随的正常粘膜。 AQP5 位于癌细胞的顶膜。另一方面，AQP3 和 AQP4 在腺癌中均没有上调。为了阐明 AQP5 在癌细胞中的作用，在低分化人胃腺癌 (MKN45) 细胞系中外源表达 AQP5。 AQP5的表达显着增加了纺锤形分化细胞的比例、碱性磷酸酶的活性（癌细胞肠上皮细胞类型的标志物）以及层粘连蛋白（上皮细胞标志物）的表达水平。用水通道蛋白抑制剂 HgCl2 处理稳定表达 AQP5 的 MKN45 细胞，可显着降低分化细胞的比例和碱性磷酸酶的活性。我们的结果表明AQP5的上调可能参与人胃癌细胞的分化。
Litttle is known about the function of aquaporin (AQP) water channels in human gastric cancer. In the upper or middle part of human stomach, we found that expression level of AQP5 protein in intestinal type of adenocarcinoma was significantly higher than that in accompanying normal mucosa. AQP5 was localized in the apical membrane of the cancer cells. On the other hand, both AQP3 and AQP4 were not up-regulated in the adenocarcinoma. To elucidate the role of AQP5 in cancer cells, AQP5 was exogenously expressed in a cell line of poorly differentiated human gastric adenocarcinoma (MKN45). The AQP5 expression significantly increased the proportion of differentiated cells with a spindle shape, the activity of alkaline phosphatase, a marker for the intestinal epithelial cell type of cancer cells, and the expression level of laminin, an epithelial cell marker. Treatment of the MKN45 cells stably expressing AQP5 with HgCl2, an inhibitor of aquaporins, significantly decreased the proportion of differentiated cells and the activity of alkaline phosphatase. Our results suggest that up-regulation of AQP5 may be involved in differentiation of human gastric cancer cells.