Two-dimensional proteome reference map for the radiation-resistant bacterium Deinococcus geothermalis
Two-dimensional proteome reference map for the radiation-resistant bacterium Deinococcus geothermalis
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DOI：
10.1002/pmic.200800657
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发表时间：
2010-02-01
期刊：
影响因子：
3.4
通讯作者：
Neubauer, Peter
中科院分区：
文献类型：
--
作者：
Liedert, Christina;Bernhardt, Joerg;Neubauer, Peter
构建了地热奇球菌胞质和细胞包膜蛋白质组的 2-DE 参考图。总共 403 个点被鉴定为 299 种不同的蛋白质。蛋白质组中独特的是 V 型 ATP 酶和奇球菌特异性蛋白质的四个亚基，构成细胞包膜蛋白质组的四分之一。细胞质蛋白质组包括中心碳代谢酶、分子伴侣、蛋白质和DNA修复酶以及氧化应激。共有34种丰富但功能未知的蛋白质可能与地热热藻的极端胁迫耐受性有关。
2-DE reference maps for Deinococcus geothermalis cytosolic and cell envelope proteomes were constructed. In total, 403 spots were identified as 299 different proteins. Unique in the proteomes were four subunits of V-type ATPase and Deinococcus specific proteins constituting one-fourth of cell envelope proteome. The cytoplasmic proteome included enzymes of the central carbon metabolism, chaperones, enzymes of protein and DNA repair, and oxidative stress. A total of 34 abundant proteins with unknown function may relate to the extreme stress tolerance of D. geothermalis.