Mitochondrial DNA variability and Wolbachia infection in two sibling woodlice species
Mitochondrial DNA variability and Wolbachia infection in two sibling woodlice species
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DOI：
10.1038/sj.hdy.6885380
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发表时间：
1999-07-01
期刊：
影响因子：
3.8
通讯作者：
Raimond, R
中科院分区：
文献类型：
--
作者：
Marcadé, I;Souty-Grosset, C;Raimond, R
几个形态学的种族和亚种已被描述，后来被纳入陆生等足类物种Porcellionides pruinosus。在我们对这个物种的研究中，我们研究了来自法国，希腊，突尼斯和留尼汪岛的标本。实验室杂交揭示了两个独立的群体：一个群体是法国人（四个地方），另一个群体是来自突尼斯、留尼汪岛和希腊的人。法国人的生殖隔离，从其他人群。我们对这7个群体的线粒体DNA（mtDNA）多态性进行了调查。我们观察到两组相应的两组人群的有丝分裂型。种群间的互育性实验和线粒体型间的遗传距离都表明存在两个不同的物种，一个在法国，一个在希腊，突尼斯和留尼汪岛。这两个物种分别拥有两条不同的沃尔巴克氏体线。分子遗传学分析的另一个特点是在法国人群中明显的线粒体单态性和其他三个群体的低变异性。这一结果可能与沃尔巴克氏体在这些人群中诱导遗传搭便车的可能性有关。
Several morphological races and subspecies have been described and later included within the terrestrial isopod species Porcellionides pruinosus. During our study of this species, we have worked on specimens from France, Greece, Tunisia and Reunion island. Laboratory crosses have revealed two separate groups of populations: French populations (four localities) in one group, and those from Tunisia, Reunion island and Greece in the other. French individuals were reproductively isolated from those of the other populations. We have undertaken a survey of mitochondrial DNA (mtDNA) polymorphism in these seven populations. We observed two groups of mitotypes corresponding to the two groups of populations. Interfertility experiments between populations and the mitochondrial genetic distances between mitotypes both suggest the presence of two different species, one in France and one in Greece, Tunisia and Reunion island. The two species harbour, respectively, two different Wolbachia lines. Another feature of the molecular genetic analysis was the apparent mitochondrial monomorphism in the French populations and the low variability in the other three populations. The result can be related to the possibility of Wolbachia-induced genetic hitchhiking in these populations.