Isolation and characterisation of Chrysanthemum crassum SOS1, encoding a putative plasma membrane Na+/H+ antiporter
Isolation and characterisation of Chrysanthemum crassum SOS1, encoding a putative plasma membrane Na+/H+ antiporter
复制标题
菊花 SOS1 的分离和表征，编码假定的质膜 Na /H 反向转运蛋白
DOI：
10.1111/j.1438-8677.2011.00560.x
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发表时间：
2012-09-01
期刊：
影响因子：
3.9
通讯作者：
Chen, F.
中科院分区：
文献类型：
--
作者：
Song, A.;Lu, J.;Chen, F.
利用简并聚合酶链反应和RACE-PCR技术，从耐盐植物菊花（Chrysanthemum crassum）中分离到SOS1（盐过敏感1）基因的全长同源基因，并编码Na+/H+反转运蛋白。3752-bp的序列包括3438 bp的开放阅读框，编码一个127-kDa的蛋白，其N端部分有12个跨膜结构域，c端部分有一个亲水的细胞质尾部。CcSOS1似乎是一种质膜蛋白，在肽水平上与其拟南芥同源物具有相似的62%的相似性。CcSOS1在根中的表达与沙蒿相似，对盐胁迫敏感，而在叶片中，CcSOS1在脱落酸存在下表达下调。CcSOS1转录本丰度在低温胁迫下的根系和叶片中均有所降低，而在干旱胁迫下，叶片中CcSOS1转录本丰度有所增加（根系中没有）。CcSOS1的表达在CaCl2存在下不受调节。酵母的异种互补实验表明，CcSOS1模仿内源性NHA1蛋白的功能，指导Na+外排。因此，CcSOS1似乎编码了盐度诱导的质膜Na+/H+反转运蛋白。该基因可能有助于利用转基因方法提高相关观赏植物的耐盐性。
A full-length cDNA homologue of SOS1 (salt overly sensitive 1) was isolated from the salinity-tolerant species Chrysanthemum crassum and found to encode a Na+/H+ antiporter, using degenerate PCR and RACE-PCR. The 3752-bp sequence comprised a 3438 bp open reading frame, encoding a 127-kDa protein with 12 transmembrane domains within its N terminal portion, and a hydrophilic cytoplasmic tail in its C-terminal portion. CcSOS1 appears to be a plasma membrane protein, and shares similar to 62% identity at the peptide level with its Arabidopsis thaliana homologue. Expression of CcSOS1 in the roots of C.similar to crassum was sensitive to salinity stress, while in the leaves CcSOS1 was down-regulated in the presence of abscisic acid. CcSOS1 transcript abundance was reduced in both roots and leaves of plants exposed to low temperature, while it was increased in leaves (but not in roots) after drought stress. CcSOS1 expression was not regulated in the presence of CaCl2. A heterologous complementation assay in yeast suggested that CcSOS1 directs Na+ efflux, mimicking the function of the endogenous NHA1 protein. Thus CcSOS1 appears to encode a salinity-inducible plasma membrane Na+/H+ antiporter. This gene may be useful in transgenic approaches to improving the salinity tolerance of related ornamental species.