Simultaneous detection of platelet-specific antibodies based on a photonic crystal-encoded suspension array
Simultaneous detection of platelet-specific antibodies based on a photonic crystal-encoded suspension array
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DOI：
10.1016/j.cca.2016.04.031
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发表时间：
2016-07-01
影响因子：
5
通讯作者：
Ling, Yun
中科院分区：
文献类型：
--
作者：
Kong, Xin;Ye, Baofen;Ling, Yun
背景：抗血小板抗体在血液中的出现是免疫性血小板减少症(ITP)的重要原因，检测血小板膜糖蛋白(GP)特异性抗体有助于ITP的诊断。方法：用抗GPIIb、-GPIIIa、-GPIb和-Gpix四种不同反射光谱的光子晶体微球包被制备成光子晶体编码悬浮阵列。同时加入异硫氰酸荧光素标记的羊抗人免疫球蛋白检测人免疫球蛋白。用荧光显微镜对检测结果进行分析。以平行Moab固定化血小板抗原(MAIPA)作为参照试验。结果：PCSA检测抗GPIIb抗体(75.0%vs31.1%)和抗GPIIa抗体(84.4%vs40.6%)的敏感性高于MAIPA(37.5%vs34.4%)和MAIPA(50.0%vs40.8%)。MAIPA和PCSA试验具有相似的特异性。PCSA检测到的含有抗GPIIa抗体或抗GPIIb抗体的血清稀释度高于MAIPA。整个检测过程控制在3.5h以内。结论：与MAIPA法相比，PCSA法具有相当或更好的灵敏度和特异度，且更快速。(C)2016爱思唯尔B.V.保留所有权利。
Background: The appearance of antibodies to platelets in the blood is an important cause of immune thrombocytopenia (ITP), and platelet glycoprotein (GP)-specific antibody detection may be helpful to diagnose this condition.Methods: Photonic crystal microspheres with different distinct reflection spectra were coated with anti-GPIIb, -GPIIIa, -GPIb and -GPIX monoclonal antibodies (MoAbs) to create a photonic crystal-encoded suspension array (PCSA). Fluorescein isothiocyanate-labelled goat anti-human IgG was added to detect human IgG simultaneously. The detection results were analysed by fluorescence microscopy. Parallel MoAb immobilization of platelet antigen (MAIPA) was used as a reference test. Both methods were used to analyse 63 clinical samples including serum from 32 ITP patients and 31 healthy humans.Results: The PCSA showed greater sensitivity than MAIPA in detecting anti-GPIIb (75.0% vs 31.1%) and GPIIIa (84.4% vs 40.6%) antibodies and similar sensitivity as MAIPA in detecting anti-GPIb (37.5% vs 34.4%) and GPIX (50.0% vs 40.8%) antibodies. The MAIPA and PCSA tests had similar specificity. The PCSA detected higher dilutions of serum containing anti-GPIIIa antibody or anti-GPIIb antibody than did MAIPA. The entire testing process was controlled within 3.5 h.Conclusions: The PCSA assay described has comparable or better sensitivity and specificity compared to the MAIPA and is more rapid. (C) 2016 Elsevier B.V. All rights reserved.