Spectroscopic and conformational study of chitosan acid salts
Spectroscopic and conformational study of chitosan acid salts
复制标题
壳聚糖酸盐的光谱和构象研究
DOI：
10.1007/s10965-008-9221-3
复制
发表时间：
2009
影响因子：
2.8
通讯作者：
P. Dutta
中科院分区：
文献类型：
--
作者：
J. Singh;P. Dutta
研究了在温和条件下以异丙醇为溶剂制备壳聚糖酸盐的方法。通过将壳聚糖分别溶解于抗坏血酸、对氨基苯甲酸和对硝基苯甲酸中，然后用异丙基/水混合溶剂洗涤来制备盐。用紫外、红外、核磁共振、X射线衍射和圆二色谱等技术对改性壳聚糖衍生物的晶盐和分子构象进行了表征。关于壳聚糖上的NH 2基团与酸上的COOH基团之间的键合，通过FTIR（1523 - 1557 cm-1）和NMR（1577 -8 ppm）证实了盐的形成。壳聚糖抗坏血酸、对氨基苯甲酸和对硝基苯甲酸盐在DMSO溶液中的圆二色性谱分别在253、285和295 nm处出现负峰，表明该聚合物具有螺旋二级结构。该聚合物在较宽的pH范围内具有高度可溶性，这为壳聚糖基材料的生物医学应用开辟了新的前景。
The efficient procedure for preparation of chitosan acid salts in isopropyl alcohol under mild condition has been demonstrated. The salts were prepared by dissolving chitosan into ascorbic acid, p-amino benzoic acid and p-nitro benzoic acid, respectively, and then washed with an isopropyl/water mix-solvent. The prepared crystal salt and molecular conformation of modified chitosan derivatives are characterized by using ultraviolet, Fourier transform infrared (FTIR),1H nuclear magnetic resonance (NMR), X-ray diffraction and Circular dichroism (CD) techniques. The formation of salts was confirmed by FTIR (∼1,523–1,557 cm−1) and NMR (∼7–8 ppm) with respect to the bonding between NH2group on chitosan and COOH group on acid. The CD spectra of chitosan salts showed negative at (253, 285 and 295 nm band) in chitosan ascorbic acid, p-amino benzoic acid and p-nitro benzoic acid salt respectively in DMSO, indicating that the polymer adopted a helical secondary structure. The polymers are highly soluble in a wide pH range, which opens new perspectives for the biomedical application of chitosan based-materials.