Disposable Emitters for On‐line Capillary Zone Electrophoresis/Nanoelectrospray Mass Spectrometry
Disposable Emitters for On‐line Capillary Zone Electrophoresis/Nanoelectrospray Mass Spectrometry
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DOI：
10.1002/(sici)1097-0231(19970215)11:3
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发表时间：
1997-02
影响因子：
2
通讯作者：
K. Bateman;Robert L. White;P. Thibault
中科院分区：
文献类型：
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作者：
K. Bateman;Robert L. White;P. Thibault
介绍了一种用于在线毛细管电泳质谱(CZE/ES-MS)、每分钟流量亚微升的一次性微喷雾器的制备新方法。提出了将这些微喷雾器连接到CZE柱上的不同方法，并与用于在线CZE/ES-MS分析的更传统的一体式毛细管装置进行了比较。使用涂有[(丙烯酰氨基)丙基]三甲基氯化铵试剂的毛细管可以提高分离性能，这是一种有吸引力的替代其他胺涂层毛细管的方法。一次性微喷雾器的使用为在线CZE/ES-MS实验提供了易操作性和灵活性，因为CZE柱可以独立于微喷雾器制备。对这些微喷雾器在CZE/ES-MS无人值守8小时以上的操作中的应用进行了评估，发现峰面积的相对标准偏差为3-8%，迁移时间的相对标准偏差小于1%。获得了全扫描质谱图，可用于低剂量的多肽Femtomol注射。应用CZE/ES-MS和CZE-串联质谱仪对菜豆中的单链凝集素进行了分析。以这种方式获得的质谱图数据提供了关于糖蛋白消化中鉴定的糖肽结构的信息。©1997加拿大皇冠版权所有
A new method for preparing disposable microsprayers, for on-line capillary-zone electrophoresis/electrospray mass spectrometry (CZE/ES-MS) at sub-microliter per minute flow rates, is described. Different means of coupling these microsprayers to the CZE columns are presented, and compared with the more traditional one-piece capillary arrangement used for on-line CZE/ES-MS analysis. Enhancement of separation performance was obtained using capillaries coated with [(acryloylamino) propyl]trimethylammonium chloride reagents, an attractive alternative to other amine-coated capillaries. The use of disposable microsprayers provided ease of operation and flexibility for on-line CZE/ES-MS experiments, since CZE columns can be prepared independently from the microsprayers. The application of these microsprayers to unattended CZE/ES-MS operation for more than 8 hours was evaluated, and found to provide relative standard deviation values of 3–8% on peak areas and less than 1% on migration times. Full-scan mass spectral acquisition was obtained for low femtomol injections of peptides. The technique was applied to the analysis of a single chain lectin from Phaseolus vulgaris, using both CZE/ES-MS and CZE combined with tandem mass spectrometry. The mass spectra data obtained in this way provided information on the structure of glycopeptides identified in digests of the glycoprotein. © 1997 Crown Copyright Canada