Developmental and cell cycle regulation of alfalfa nucMs1, a plant homolog of the yeast Nsr1 and mammalian nucleolin
Developmental and cell cycle regulation of alfalfa nucMs1, a plant homolog of the yeast Nsr1 and mammalian nucleolin
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DOI：
10.1105/tpc.8.3.417
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发表时间：
1996-03-01
期刊：
影响因子：
11.6
通讯作者：
Hirt, H
中科院分区：
文献类型：
--
作者：
Bogre, L;Jonak, C;Hirt, H
我们在此报告了 nucMs1 苜蓿 cDNA 的分离和表征，其预测的氨基酸序列在结构上类似于酵母 Nsr1 蛋白和动物核蛋白。这些蛋白质由 N 端酸性结构域、位于中心的 RNA 识别基序 (RRM) 以及 C 端富含甘氨酸和精氨酸的结构域组成。与含有四个 RRM 的动物核蛋白相比，NucMs1 更类似于仅含有两个 RRM 的酵母 Nsr1 蛋白。 NucMs1 C 端肽抗体特异性识别苜蓿细胞中的 95 kD 核仁蛋白，该蛋白以细胞周期依赖性方式改变其定位。 nucMs1 转录物和 p95(nucMs1) 蛋白水平与细胞增殖相关，并且发现在 G(0) 停滞的叶细胞的有丝分裂刺激后，nucMs1 基因表达在 G(1) 期被诱导。对不同发育阶段苜蓿不同器官的原位杂交分析表明，nucMs1基因在根分生组织细胞中表达量最高，但在植物体的其他分生组织细胞中也有发现，nucMs1表达与细胞增殖紧密相关，但不依赖于特定的细胞周期阶段。在已退出细胞周期并正在进行分化或极生长的细胞中未观察到 nucMs1 表达，表明 nucMs1 对于细胞增殖以外的过程可能不是必需的。
We report here the isolation and characterization of the nucMs1 alfalfa cDNA, whose predicted amino acid sequence structurally resembles the yeast Nsr1 protein and animal nucleolins. These proteins consist of an N-terminal acidic domain, centrally located RNA recognition motifs (RRMs), and a C-terminal glycine- and arginine-rich domain. In comparison with animal nucleolins that contain four RRMs, NucMs1 more closely resembles the yeast Nsr1 protein, which contains only two RRMs. A NucMs1 C-terminal peptide antibody specifically recognized a 95-kD nucleolar protein in alfalfa cells that changed its localization in a cell cycle-dependent manner. The nucMs1 transcript and p95(nucMs1) protein levels correlated with cell proliferation, and nucMs1 gene expression was found to be induced in the G(1) phase upon mitogenic stimulation of G(0)-arrested leaf cells. In situ hybridization analysis of different alfalfa organs during various developmental stages showed that nucMs1 gene expression is highest in root meristematic cells, but it is also found in other meristematic cells of the plant body, nucMs1 expression is tightly linked to cell proliferation but does not depend on a particular cell cycle phase. No nucMs1 expression was observed in cells that had exited the cell cycle and were undergoing differentiation or polar growth, indicating that nucMs1 may not be necessary for processes other than cell proliferation.