Direct interaction between gingival fibroblasts and lymphoid cells induces inflammatory cytokine mRNA expression in gingival fibroblasts
Direct interaction between gingival fibroblasts and lymphoid cells induces inflammatory cytokine mRNA expression in gingival fibroblasts
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DOI：
10.1177/00220345990780011001
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发表时间：
1999-01-01
影响因子：
7.6
通讯作者：
Okada, H
中科院分区：
文献类型：
--
作者：
Murakami, S;Hino, E;Okada, H
在发炎的牙周病变中，通常在牙龈成纤维细胞附近的血管外牙周结缔组织中观察到淋巴细胞的密集浸润。我们前期的研究表明，活化的淋巴细胞在体外可以与牙龈成纤维细胞发生特异性的相互作用。在本研究中，我们调查牙龈成纤维细胞是否通过直接与淋巴细胞的相互作用，通过监测炎症细胞因子mRNA在人牙龈成纤维细胞（HGF）的表达被激活。在体外与各种人淋巴样细胞共培养导致HGF表达IL-1 α、IL-1 β和IL-6 mRNA显著增加。此外，在HGF中组成性地观察到IL-1 β转化酶（ICE）的mRNA表达，其对于产生成熟形式的IL-1 β是必需的，表明成熟IL-1 β由这些细胞产生。当HGF与淋巴细胞的培养上清液一起培养时，未观察到炎性细胞因子mRNA表达的增加。类似地，当HGF和淋巴样细胞在同一孔中培养但被防止细胞之间直接接触的膜隔开时，未观察到炎性细胞因子mRNA表达的增加。这些结果强烈地表明，这些异型细胞类型之间的直接相互作用将激活信号转导到HGF中，从而诱导炎性细胞因子mRNA表达的增加。此外，当HGF在外源性IL-1 β存在下直接与淋巴细胞相互作用时，HGF中IL-1 β mRNA的表达协同增加。本研究表明，HGF和淋巴细胞之间的直接相互作用刺激HGF增加炎症细胞因子mRNA的表达，并提出了异型细胞-细胞相互作用可能促进局部炎症反应的可能性。
In inflamed periodontal lesions, dense infiltration of lymphocytes is usually observed in the extravascular periodontal connective tissue, adjacent to gingival fibroblasts. Our previous study revealed that activated lymphocytes can adhesively interact with gingival fibroblasts in vitro. In the present study, we investigated whether gingival fibroblasts are activated through direct interaction with lymphoid cells by monitoring the expression of inflammatory cytokine mRNA in human gingival fibroblasts (HGF). Co-culture with various human lymphoid cells in vitro resulted in a marked increase in the expression of IL-1 alpha, IL-1 beta, and IL-6 mRNA by the HGF. In addition, expression of the mRNA of the IL-1 beta-converting enzyme (ICE), which is essential to produce the mature form of IL-1 beta, was constitutively observed in the HGF, suggesting that mature IL-1 beta is produced by these cells. When HGF were cultured with the culture supernatant of the lymphoid cells, the increase in the inflammatory cytokine mRNA expression was not observed. Similarly, when HGF and lymphoid cells were cultured in the same well but separated by a membrane which prevented direct contact between the cells, no increase in inflammatory cytokine mRNA expression was observed. These results strongly indicate that direct interaction between these heterotypic cell types transduces activation signals into HGF that induce an increase in inflammatory cytokine mRNA expression. Furthermore, IL-1 beta mRNA expression in the HGF was synergistically increased when HGF directly interacted with lymphoid cells in the presence of exogeneous IL-1 beta. The present study demonstrates that direct interaction between HGF and lymphoid cells stimulates HGF to increase inflammatory cytokine mRNA expression, and raises the possibility that heterotypic cell-cell interaction may facilitate local inflammatory reactions.