Autophagy mediates a direct synergistic interaction during co-transmission of two distinct arboviruses by insect vectors
Autophagy mediates a direct synergistic interaction during co-transmission of two distinct arboviruses by insect vectors
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DOI：
10.1007/s11427-022-2228-y
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发表时间：
2023-03
期刊：
影响因子：
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通讯作者：
Dongsheng Jia;Qifu Liang;Hongyan Chen;Huan Liu;Guangjun Li;Xiao-Feng Zhang;Qian Chen;Aiming Wang
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文献类型：
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作者：
Dongsheng Jia;Qifu Liang;Hongyan Chen;Huan Liu;Guangjun Li;Xiao-Feng Zhang;Qian Chen;Aiming Wang
多种病毒协同感染昆虫是自然界中常见的现象，但其潜在机制仍不清楚。在这里，我们发现，水稻瘤矮呼肠孤病毒（RGDV）促进水稻条纹花叶弹状病毒（RSMV）的传播共感染叶蝉载体。RSMV核蛋白（N）单独激活完全抗病毒自噬，而RGDV非结构蛋白Pns 11单独诱导前病毒不完全自噬。在共感染的载体中，RSMV利用Pns 11诱导的自噬体通过N-Pns 11-ATG 5相互作用组装包膜病毒体。RSMV在Sf 9细胞中能有效增殖。Pns 11在Sf 9细胞或叶蝉载体中的表达引起N从ER到Pns 11诱导的自噬体的募集，并抑制N诱导的完全自噬通量，最终促进RSMV繁殖。总之，这些结果表明，在两种不同的虫媒病毒通过昆虫载体共传播期间，自噬在调节直接协同相互作用中的作用是以前未被认识到的，并揭示了病毒诱导的自噬体在弹状病毒组装中的功能重要性。
Multiple viral infections in insect vectors with synergistic effects are common in nature, but the underlying mechanism remains elusive. Here, we find that rice gall dwarf reovirus (RGDV) facilitates the transmission of rice stripe mosaic rhabdovirus (RSMV) by co-infected leafhopper vectors. RSMV nucleoprotein (N) alone activates complete anti-viral autophagy, while RGDV nonstructural protein Pns11 alone induces pro-viral incomplete autophagy. In co-infected vectors, RSMV exploits Pns11-induced autophagosomes to assemble enveloped virions via N-Pns11-ATG5 interaction. Furthermore, RSMV could effectively propagate in Sf9 cells. Expression of Pns11 in Sf9 cells or leafhopper vectors causes the recruitment of N from the ER to Pns11-induced autophagosomes and inhibits N-induced complete autophagic flux, finally facilitating RSMV propagation. In summary, these results demonstrate a previously unappreciated role of autophagy in the regulation of the direct synergistic interaction during co-transmission of two distinct arboviruses by insect vectors and reveal the functional importance of virus-induced autophagosomes in rhabdovirus assembly.