IDENTIFICATION OF VCR, A REPEATED SEQUENCE ASSOCIATED WITH A LOCUS ENCODING A HEMAGGLUTININ IN VIBRIO-CHOLERAE O1
IDENTIFICATION OF VCR, A REPEATED SEQUENCE ASSOCIATED WITH A LOCUS ENCODING A HEMAGGLUTININ IN VIBRIO-CHOLERAE O1
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DOI：
10.1128/jb.176.17.5450-5458.1994
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发表时间：
1994-09-01
影响因子：
3.2
通讯作者：
MANNING, PA
中科院分区：
文献类型：
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作者：
BARKER, A;CLARK, CA;MANNING, PA
我们已经确定了霍乱弧菌569 B染色体的6.3-kb BamHI片段的核苷酸序列，该片段包括以前报道的甘露糖-岩藻糖抗性血凝素的序列（V.L. Franzon，A. Barker和P.A.曼宁感染Immun. 61：3032-3037，1993）。该区域含有9个拷贝的124 bp的不完全二分体对称的同向重复序列（此处称为VCR），Southern杂交显示其在霍乱弧菌01染色体中至少出现60至100次。大规模的染色体作图表明，重复序列仅限于染色体的约10%。相关序列也在非01霍乱弧菌中发现，但在弧菌科的其他成员中没有发现。然而，VCR与先前描述的其他重复序列无关。
We have determined the nucleotide sequence of a 6.3-kb BamHI fragment of the chromosome of Vibrio cholerae 569B that includes the sequence of the mannose-fucose-resistant hemagglutinin reported previously (V. L. Franzon, A. Barker, and P. A. Manning, Infect. Immun. 61:3032-3037, 1993). This region contains nine copies of a 124-bp direct repeat, here named VCR, of imperfect dyad symmetry, that are shown by Southern hybridization to occur at least 60 to 100 times in the V. cholerae 01 chromosome. Large-scale chromosomal mapping suggests that the repeats are confined to about 10% of the chromosome. Related sequences are also found in non-01 V. cholerae but not in other members of the family Vibrionaceae. However, VCR is unrelated to other previously described repetitive sequences.