Production of human papillomavirus type 16 E7 protein in Lactococcus lactis
Production of human papillomavirus type 16 E7 protein in Lactococcus lactis
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DOI：
10.1128/aem.68.2.917-922.2002
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发表时间：
2002-02-01
影响因子：
4.4
通讯作者：
Le Loir, Y
中科院分区：
文献类型：
--
作者：
Bermúdez-Humarán, LG;Langella, P;Le Loir, Y
人乳头瘤病毒16型E7蛋白在乳酸乳球菌中产生。分泌允许更高的生产产量比细胞质生产。在稳定期，细胞质E7的量减少，而分泌的E7的量增加，这表明一个阶段依赖性的细胞内蛋白水解。E7与葡萄球菌核酸酶（一种稳定的蛋白质）的融合产生高度稳定的胞质蛋白。这项工作为宫颈癌病毒筛查系统和口服疫苗的发展提供了新的候选人。
The E7 protein of human papillomavirus type 16 was produced in Lactococcus lactis. Secretion allowed higher production yields than cytoplasmic production. In stationary phase, amounts of cytoplasmic E7 were reduced, while amounts of secreted E7 increased, suggesting a phase-dependent intracellular proteolysis. Fusion of E7 to the staphylococcal nuclease, a stable protein, resulted in a highly stable cytoplasmic protein. This work provides new candidates for development of viral screening systems and for oral vaccine against cervical cancer.