Preferential organ attachment and invasion in vitro by B16 melanoma cells selected for differing metastatic colonization and invasive properties.
Preferential organ attachment and invasion in vitro by B16 melanoma cells selected for differing metastatic colonization and invasive properties.
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根据不同的转移定植和侵袭特性选择的 B16 黑色素瘤细胞在体外优先附着和侵袭器官。
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发表时间：
1985
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影响因子：
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通讯作者：
D. Welch
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作者：
G. Nicolson;K. Dulski;C. Basson;D. Welch
小鼠B16黑色素瘤亚系在体内先后被选为低(B16-F1)或高(B16-F10)肺、高卵巢(B16-O10)或高脑(B16-B15b)定植，以及体外增强组织侵袭(B16-BL6)。这些B16亚系在体外进行了同种器官黏附/侵袭试验，以确定肿瘤和/或宿主组织特性的差异，这可能是某些部位优先转移的原因。所用组织为C57BL/6小鼠的肺、卵巢和心脏。在8个独立的实验中，高肺定植的B16-F10细胞与肺组织的结合和渗透好于卵巢或心脏组织，而高卵巢定植的B16-O10细胞附着和侵袭卵巢组织的比率高于肺或心脏组织。在实验中，只有高组织侵袭性的B16-BL6细胞能够在18小时内侵入心脏组织。这些结果表明，恶性肿瘤细胞对器官转移的定植偏好可能部分取决于转移的肿瘤细胞附着和侵袭靶组织的能力的差异。
Murine B16 melanoma sublines have been sequentially selected in vivo for low (B16-F1) or high (B16-F10) lung, high ovary (B16-O10) or high brain (B16-B15b) colonization, and in vitro for enhanced tissue invasion (B16-BL6). These B16 sublines were tested in vitro in a syngeneic organ adhesion/invasion assay to determine differences in tumor and/or host tissue properties that might account for preferential metastasis to certain sites. Tissues used were murine C57BL/6 lung, ovary and heart. In 8 independent experiments high lung-colonizing B16-F10 cells bound to and infiltrated into lung tissue better than ovary or heart tissue, while high ovary-colonizing B16-O10 cells attached to and invaded into ovary tissue at higher rates than lung or heart tissue. Only highly tissue-invasive B16-BL6 cells were able to invade heart tissue within 18 h in the experiments. The results suggest that organ metastatic colonization preferences by malignant cells may be determined, in part, by differences in the abilities of metastatic tumor cells to attach to and invade target tissue.