Embryonic precancerous and cancerous human antigens recognized by monoclonal antibodies.
Embryonic precancerous and cancerous human antigens recognized by monoclonal antibodies.
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单克隆抗体识别胚胎癌前和癌性人类抗原。
DOI：
10.1002/9780470720776.ch12
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Koprowski,H
中科院分区：
文献类型：
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作者：
Koprowski,H
人黑素瘤小鼠免疫后产生的单克隆抗体定义的蛋白抗原不仅在黑素瘤中表达，也在其他神经嵴源性肿瘤（如星形细胞瘤和神经母细胞瘤）中表达。其他单克隆抗体与黑色素瘤和胎儿而非成人黑色素细胞表达的抗原反应。普通痣和癌前病变（如发育不良痣）的细胞与黑色素瘤有许多相同的抗原，但与正常的黑色素细胞没有相同的抗原。与黑色素瘤。组织培养的Naevi具有寿命有限的特点。在wivo发育不良痣的恶性转化中起作用的因素，在组织培养系统中明显缺乏，目前正在研究中。用人胃肠道癌细胞免疫小鼠后产生的单克隆抗体定义糖脂抗原。其中一种抗原的碳水化合物结构是乳- n -fucopentaose 111。这种抗原在各种人类肿瘤中都有很强的表达，在免疫过氧化物酶试验中，相应的单克隆抗体也能与正常的人类上皮结合。另一种单唾液神经节脂苷是一种抗原，可在胃肠道肿瘤细胞如胰腺、胃和大肠以及结肠绒毛腺瘤细胞中表达，但不能在正常结肠粘膜细胞中表达。它的碳水化合物是一种唾液化的叶活性五糖（唾液化乳酸- n -fucopentaose 11）。一种迄今未被发现的唾液基转移酶可能参与Lewis A糖脂合成这种抗原，可能在肿瘤组织和胚胎发生的早期阶段活跃，但在正常成人组织中不活跃。
Monoclonal antibodies produced after immunization of mice with human melanomas define protein antigens expressed not only by melanomas hut also by other tunours of neural crest origin such as astrocytomas and neuroblastomas. Other monoclonal antibodies react with antigens expressed by melanomas and fetal but not adult human melanocytes. Cells of common naevi and of precancerous lesions such as dysplastic naevi share many antigens with melanomas but not with normal melanocytes. Unlike melanomas. naevi in tissue culture are characterized by a finite lifetime. Factors that are instrumental in malignant transformation of dysplastic naevi in wivo and are apparently lacking in the tissue culture system are currently under study. Monoclonal antibodies produced after immunizing mice with cells of human gastrointestinal carcinomas define glycolipid antigens. The carbohydrate structure of one of these antigens is lacto-N-fucopentaose 111. This antigen is very strongly expressed by a varietv of human tumours and in the immunoperoxidase assay the respective monoclonal antihody also binds to normal human epithelium.Another monosialoganglioside is an antigen expressed by cells of gastrointestinal tract tumours such as of the pancreas, stomach and large bowel and by cells of villous adenomas of the colon, but not by cells of normal colonic mucosa. Its carbohydrate is a sialylated Lea-active pentasaccharide (sialylated lacto-N-fucopentaose 11). A hitherto undiscovered sialyltransferase, which may be involved in synthesis of this antigen from Lewis A glycolipid, is probably active in tumour tissue and at early stages ofembrvogenesis but not in normal adult tissue.