The GW2-WG1-OsbZIP47 pathway controls grain size and weight in rice
The GW2-WG1-OsbZIP47 pathway controls grain size and weight in rice
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DOI：
10.1016/j.molp.2021.04.011
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发表时间：
2021-08-02
期刊：
影响因子：
27.5
通讯作者：
Li, Yunhai
中科院分区：
文献类型：
--
作者：
Hao, Jianqin;Wang, Dekai;Li, Yunhai
种子大小的调控是提高作物产量的关键策略，也是一个基本的生物学问题。然而，植物决定其种子大小的分子机制仍然难以捉摸。在这里，我们报告，GW 2-WG 1-OsbZIP 47调控模块控制水稻的粒宽和粒重。WG 1编码谷氧还蛋白，通过增加细胞增殖促进谷物生长。有趣的是，WG 1与转录因子OsbZIP 47相互作用，并通过与转录辅阻遏物ASP 1结合来抑制其转录活性，表明WG 1可能作为衔接蛋白来募集转录辅阻遏物。相反，OsbZIP 47通过减少细胞增殖来限制晶粒生长。进一步的研究表明，E3泛素连接酶GW 2泛素化WG 1并靶向其降解。遗传分析证实GW 2、WG 1和OsbZIP 47在控制谷粒生长的共同途径中起作用。综上所述，我们的研究结果揭示了GW 2-WG 1-OsbZIP 47调控模块控制籽粒大小和重量的遗传和分子框架，为改善作物种子大小和重量提供了新的目标。
Regulation of seed size is a key strategy for improving crop yield and is also a basic biological question. However, the molecular mechanisms by which plants determine their seed size remain elusive. Here, we report that the GW2-WG1-OsbZIP47 regulatory module controls grain width and weight in rice. WG1, which encodes a glutaredoxin protein, promotes grain growth by increasing cell proliferation. Interestingly, WG1 interacts with the transcription factor OsbZIP47 and represses its transcriptional activity by associating with the transcriptional co-repressor ASP1, indicating that WG1 may act as an adaptor protein to recruit the transcriptional co-repressor. In contrary, OsbZIP47 restricts grain growth by decreasing cell proliferation. Further studies reveal that the E3 ubiquitin ligase GW2 ubiquitinates WG1 and targets it for degradation. Genetic analyses confirm that GW2, WG1, and OsbZIP47 function in a common pathway to control grain growth. Taken together, our findings reveal a genetic and molecular framework for the control of grain size and weight by the GW2-WG1-OsbZIP47 regulatory module, providing new targets for improving seed size and weight in crops.