Rutin Inhibits Streptococcus suis Biofilm Formation by Affecting CPS Biosynthesis.
Rutin Inhibits Streptococcus suis Biofilm Formation by Affecting CPS Biosynthesis.
复制标题
芦丁通过影响 CPS 生物合成抑制猪链球菌生物膜形成
DOI：
10.3389/fphar.2017.00379
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发表时间：
2017
影响因子：
5.6
通讯作者：
Li Y
中科院分区：
文献类型：
--
作者：
Wang S;Wang C;Gao L;Cai H;Zhou Y;Yang Y;Xu C;Ding W;Chen J;Muhammad I;Chen X;He X;Liu D;Li Y
猪链球菌（Streptococcus suis，S. suis）形成生物膜并在人和猪中引起严重疾病。生物膜是包埋在细胞外聚合物基质中的微生物群落。使用抗生素或杀生物剂消除生物膜通常是无效的，需要用其他潜在的试剂替代。与传统药剂相比，有前途和潜在的替代品是不损害生长的生物膜抑制化合物。在这里，我们筛选了一个S。猪粘附抑制剂，芸香苷，来源于丁香属。大黄素是一种黄酮类化合物，对S.不影响其生长。据报道，荚膜多糖（CPS）参与其粘附以影响细菌生物膜的形成。研究了芦丁对S. CPS的内容和结构。结果表明，芦丁有利于提高S.不改变其结构。我们进一步提供的证据表明，芦丁特别影响S。通过影响CPS的生物合成来提高猪生物被膜的敏感性。研究了芦丁对S.可用作粘附抑制剂以预防S.猪生物膜相关感染。芦丁可作为一种新型的生物膜天然抑制剂，其分子机制为其药理和临床应用提供了依据。
Streptococcus suis (S. suis) form biofilms and causes severe diseases in humans and pigs. Biofilms are communities of microbes embedded in a matrix of extracellular polymeric substances. Eradicating biofilms with the use of antibiotics or biocides is often ineffective and needs replacement with other potential agents. Compared to conventional agents, promising and potential alternatives are biofilm-inhibiting compounds without impairing growth. Here, we screened a S. suis adhesion inhibitor, rutin, derived from Syringa. Rutin, a kind of flavonoids, shows efficient biofilm inhibition of S. suis without impairing its growth. Capsular polysaccharides(CPS) are reported to be involved in its adherence to influence bacterial biofilm formation. We investigated the effect of rutin on S. suis CPS content and structure. The results showed that rutin was beneficial to improve the CPS content of S. suis without changing its structure. We further provided evidence that rutin specifically affected S. suis biofilm susceptibility by affecting CPS biosynthesis in vitro. The study explores the antibiofilm potential of rutin against S. suis which can be used as an adhesion inhibitor for the prevention of S. suis biofilm-related infections. Nevertheless, rutin could be used as a novel natural inhibitor of biolfilm and its molecular mechanism provide basis for its pharmacological and clinical applications.