Identification of conserved and novel miRNAs responsive to heat stress in flowering Chinese cabbage using high-throughput sequencing
Identification of conserved and novel miRNAs responsive to heat stress in flowering Chinese cabbage using high-throughput sequencing
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DOI：
10.1038/s41598-019-51443-y
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发表时间：
2019-10-17
影响因子：
4.6
通讯作者：
Guo, Peiguo
中科院分区：
文献类型：
--
作者：
Ahmed, Waqas;Xia, Yanshi;Guo, Peiguo
植物microRNAs （miRNAs）是非编码的内源关键调控因子，在调控植物对逆境的反应和生长发育中发挥重要作用。热胁迫是影响开花大白菜产量和品质的重要非生物胁迫。中华植物变种(Tsen et Lee)。然而，关于mirna是否参与油菜芽孢杆菌热应激调节的信息有限。利用38℃高温胁迫后0 h、1 h、6 h和12 h收集的大白菜叶片，采用高通量测序方法在4个小RNA文库中鉴定出新的、保守的热响应性mirna。分析共鉴定出41个保守mirna（属于19个MIR家族），其中MIR156、MIR159、MIR168、MIR171和MIR1885的分子丰度最高。使用未注释的reads预测和评估新的mirna，得到18个候选mirna。差异表达分析显示，大多数鉴定的mirna在热处理组中下调。为了更好地理解功能的重要性，生物信息学分析预测了432种独特的推测目标mirna，这些mirna参与细胞、细胞部分、催化活性、细胞过程和非生物应激反应。此外，《京都基因百科全书》和开花白菜基因组图谱发现，mirna在胁迫适应和胁迫耐受以及包括细胞死亡在内的几种丝裂原激活蛋白激酶信号通路中发挥着重要作用。本文对这些mirna进行了全面的研究，以了解它们在开花白菜热胁迫中的调控机制及其参与。
Plant microRNAs (miRNAs) are noncoding and endogenous key regulators that play significant functions in regulating plant responses to stress, and plant growth and development. Heat stress is a critical abiotic stress that reduces the yield and quality of flowering Chinese cabbage (Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee). However, limited information is available on whether miRNAs are involved in the regulation of heat stress in B. campestris. A high-throughput sequencing approach was used to identify novel and conserved heat-responsive miRNAs in four small RNA libraries of flowering Chinese cabbage using leaves collected at 0 h,1 h, 6 h and 12 h after a 38 degrees C heat-stress treatment. The analysis identified 41 conserved miRNAs (belonging to 19 MIR families), of which MIR156, MIR159, MIR168, MIR171 and MIR1885 had the most abundant molecules. Prediction and evaluation of novel miRNAs using the unannotated reads resulted in 18 candidate miRNAs. Differential expression analysis showed that most of the identified miRNAs were downregulated in heat-treated groups. To better understand functional importance, bioinformatic analysis predicted 432 unique putative target miRNAs involved in cells, cell parts, catalytic activity, cellular processes and abiotic stress responses. Furthermore, the Kyoto Encyclopedia of Genes and Genomes maps of flowering Chinese cabbage identified the significant role of miRNAs in stress adaptation and stress tolerance, and in several mitogen-activated protein kinases signaling pathways including cell death. This work presents a comprehensive study of the miRNAs for understanding the regulatory mechanisms and their participation in the heat stress of flowering Chinese cabbage.