Genomic analysis of Ovis aries (Ovar) MHC class IIa loci
Genomic analysis of Ovis aries (Ovar) MHC class IIa loci
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DOI：
10.1007/s00251-008-0275-5
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发表时间：
2008-04-01
期刊：
影响因子：
3.2
通讯作者：
Knowles, Donald P.
中科院分区：
文献类型：
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作者：
Herrmann-Hoesing, Lynn M.;White, Stephen N.;Knowles, Donald P.
确定绵羊（Ovar）的主要组织相容性复合物IIa类区域的基因组组织是未来的抗原呈递相关的功能研究所必需的。本研究以朗布依埃公羊外周血白细胞（PBL）DNA为材料，构建了细菌人工染色体（BAC）文库，并对主要组织相容性复合体（MHC）Ⅱ类DQB2、DQA2、DQB1、DQA1和DRB 1基因座的BAC克隆进行了鉴定和全序列测定。BAC克隆由160，889bp的完成序列组成，其中基因座按以下顺序排列：DQB 2、DQA 2、DQB 1、DQA 1和DRB 1，基因座之间的跨度分别为14.3、25、6.6和40.9Kb。所有这五个基因座在用于产生MHC II类BAC克隆的动物中转录。在序列中鉴定了重复序列或逆转录转座因子沿着与由S、X和Y盒组成的MHC II类顺式启动子元件。此外，在灵长类、食肉动物和反刍动物中还发现了16个非编码保守序列（p
Determining the genomic organization of the Ovis aries (Ovar) major histocompatibility complex class IIa region is essential for future functional studies related to antigen presentation. In this study, a bacterial artificial chromosome (BAC) library of genomic DNA from peripheral blood leukocytes (PBL) of a Rambouillet ram was constructed, and BAC clone consisting of the major histocompatibility complex (MHC) class II DQB2, DQA2, DQB1, DQA1, and DRB1 loci was identified and completely sequenced. The BAC clone consists of 160,889 bp of finished sequence with the loci arranged in the following order: DQB2, DQA2, DQB1, DQA1, and DRB1 with 14.3, 25, 6.6, and 40.9 Kb spanning between the loci, respectively. All five of these loci were transcribed in the animal used to generate the MHC class II BAC clone. Repeat or retrotransposable elements along with MHC class II cis promoter elements consisting of S, X, and Y boxes were identified in the sequence. In addition, 16 non-coding conserved sequences amongst primates, carnivores, and ruminants were identified (p