Effects of Taurine and L-Arginine on the Apoptosis of Vascular Smooth Muscle Cells in Insulin Resistance Hypertensive Rats
Effects of Taurine and L-Arginine on the Apoptosis of Vascular Smooth Muscle Cells in Insulin Resistance Hypertensive Rats
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DOI：
10.1007/978-94-024-1079-2_63
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发表时间：
2017-01-01
期刊：
影响因子：
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通讯作者：
Hu, Jianmin
中科院分区：
文献类型：
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作者：
Liang, Weiwei;Yang, Qunhui;Hu, Jianmin
目的：探讨牛磺酸和L-精氨酸对胰岛素抵抗高血压大鼠血管平滑肌细胞凋亡的影响及其机制。方法：采用25%果糖灌胃12周的方法建立大鼠胰岛素抵抗性高血压模型。TUNEL法检测VSMCs凋亡。免疫组化法检测Bax蛋白和Bcl-2蛋白的表达。结果：高浓度果糖可显著抑制VSMCs凋亡。牛磺酸和L-精氨酸通过上调Bax蛋白表达和下调Bcl-2蛋白表达促进VSMCs凋亡。结论：牛磺酸和L-精氨酸对胰岛素抵抗高血压大鼠有明显的降压作用，其机制可能与促进血管平滑肌细胞凋亡，减轻血管重构有关。此外，牛磺酸和L-精氨酸联合用药比单用更有效。
Objective: In order to find the effects and mechanism of taurine and L-arginine on the apoptosis of VSMCs in insulin resistance hypertensive rats. Methods: 25% fructose were administered in the drinking water to Wistar rats for 12 weeks to induce the insulin resistance hypertensive model. Apoptosis of VSMCs was identified by TUNEL. The expressions of Bax protein and Bcl-2 protein were examined by immunohistochemistry. Results: High level of fructose significantly suppressed the apoptosis of VSMCs. Taurine and L-arginine promoted apoptosis of VSMCs via increasing the Bax protein expression and decreasing the Bcl-2 protein expression. Conclusions: Taurine and l-arginine have obvious anti-hypertensive effects in insulin resistance hypertensive rats, and its mechanism might partially be associated with attenuating vascular remodeling by promoting apoptosis in VSMCs. In addition, the combined medication of taurine and l-arginine was more effective than single medication.