Quartz crystal microbalance sensor design I.: Experimental study of sensor response and performance
Quartz crystal microbalance sensor design I.: Experimental study of sensor response and performance
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DOI：
10.1016/j.snb.2006.07.027
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发表时间：
2007-04-10
影响因子：
8.4
通讯作者：
Aastrup, Teodor
中科院分区：
文献类型：
--
作者：
Anderson, Henrik;Jonsson, Mats;Aastrup, Teodor
本文研究了一种新型的石英晶体微天平(QCM)生物传感器，该传感器由一个矩形的小流量池和一个相应形状的晶体电极组成。通过阻抗分析对该传感器进行了评价，并与标准圆形传感器晶体和小圆电极传感器晶体进行了比较。在矩形和标准圆形传感器系统上对抗体-抗原相互作用系统进行了QCM比较测量。三种不同传感器晶体的阻抗分析和随后的数据提取表明，较小的传感器在空气中具有明显较高的Q值，但电极上的液体负载从根本上降低了所有晶体的Q值。在液体载荷作用下，标准圆形和矩形传感器的Q值相近，而小型圆形传感器的Q值高出50%。QCM实验表明，矩形晶体电极的QCM系统在液体环境中是完全可以工作的。矩形系统对一系列抗体注射表现出更高和更快的反应，尽管噪音水平高于标准系统。这项研究阐明了通过优化传感器电极的大小和形状以及流动池的几何形状来提高传感器性能的巨大潜力。(C)2006爱思唯尔B.V.保留所有权利。
This paper investigates a novel quartz crystal microbalance (QCM) biosensor with a small and rectangular flow cell along with a correspondingly shaped crystal electrode. The sensor was evaluated with impedance analysis and compared to standard circular sensor crystals and sensor crystals with small circular electrodes. Comparative QCM measurements on an antibody-antigen interaction system were carried out on the rectangular and standard circular sensor systems. Impedance analysis and subsequent data extraction of the three different sensor crystals showed that the smaller sensors had significantly higher Q-values in air, but that liquid load on the electrodes lowered the Q-values radically for all crystals. Under liquid load, Q-values for the standard circular and the rectangular sensors were similar whereas the Q-value for the small circular sensor was 50% higher. QCM experiments showed that the QCM system with rectangular crystal electrodes was fully functional in a liquid environment. The rectangular system showed higher and more rapid responses for series of antibody injections, albeit at a higher noise level than the standard system. The study elucidates a significant potential for improvement of sensor performance by optimising the sensor electrode size and shape together with the flow cell geometry. (c) 2006 Elsevier B.V. All rights reserved.