Some additional data to the occurrence, morphology and validity of Myxobolus turpisrotundus Zhang, 2009 (Myxozoa: Myxosporea)
Some additional data to the occurrence, morphology and validity of Myxobolus turpisrotundus Zhang, 2009 (Myxozoa: Myxosporea)
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DOI：
10.1007/s00436-010-1835-9
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发表时间：
2010-06-01
影响因子：
2
通讯作者：
Luo, Y. L.
中科院分区：
文献类型：
--
作者：
Liu, Y.;Gu, Z. M.;Luo, Y. L.
自1955年在中国金鱼Carassius auratus auratus （L.）中首次报道以来，张氏粘虫（Myxobolus turpisrotundus Zhang, 2009）感染异种发生的异种鲫Carassius auratus gibelio (Bloch)，一直被认为与Nemeczek， 1911同义。本研究采用形态学和分子生物学方法对其进行了全面的研究。圆芽孢杆菌的圆形孢子在形态上与布兰的圆芽孢杆菌相似；然而，我们在形态学上发现了细微的差异。孢子的长度和宽度与极蒴果的长度和宽度之比通常在2.0和1.9以下，而圆轮霉的长度和宽度与极蒴果的长度和宽度之比通常在2.0和1.9以上。圆轮田鼠的疟原虫直径为600 ~ 6200亩，圆轮田鼠的疟原虫直径为60 ~ 180亩。扫描观察结果显示，疣状芽孢杆菌孢子表面普遍呈凹坑状。然而，圆形霉菌的表面是光滑的。序列比较发现，圆齿圆齿霉小亚基核核体RNA基因序列与已发表的圆齿圆齿霉序列不匹配（EU710583, 85%大于742 bp； FJ851447, 85%大于742 bp； FJ851448, 85%大于742 bp； FJ851449, 85%大于742 bp）。此外，系统发育分析表明，圆尾圆尾与异基因异育银鲫的高自举值（100% neighbor-joining， NJ; 100% maximum parsimony， MP）聚在一起，与鲷鱼的高自举值（100% NJ, 100% MP）聚在一起。从形态学和分子生物学数据上，我们获得了足够的证据来支持turpisrotundus的有效性。
Myxobolus turpisrotundus Zhang, 2009, infects allogynogenetic gibel carp Carassius auratus gibelio (Bloch) and is always regarded as synonymous with Myxobolus rotundus Nemeczek, 1911, since its first report in goldfish Carassius auratus auratus (L.) in China in 1955. In this study, it was comprehensively examined by morphological and molecular biological methods. The round spores of M. turpisrotundus are similar to those of M. rotundus from common bream Abramis brama (L.) in morphology; however, we detected slight differences in morphometry. The ratios of the length and width of the spore to the length and width of the polar capsule of M. turpisrotundus are usually below 2.0 and 1.9, respectively, however these ratios are always above 2.0 and 1.9 in M. rotundus. The plasmodium size of M. turpisrotundus is 600-6,200 mu m in diameter and that of M. rotundus is 60-180 mu m in diameter. Scanning observation showed the spore surface of M. turpisrotundus was generally pitted. Yet the surface of M. rotundus is smooth. Sequence comparison revealed the small subunit ribosomal RNA gene sequence of M. turpisrotundus did not match any published sequences of M. rotundus (EU710583, 85% over 742 bp; FJ851447, 85% over 742 bp, FJ851448, 85% over 742 bp; FJ851449, 85% over 742 bp). Moreover, phylogenetic analysis showed M. turpisrotundus clustered with the species from allogynogenetic gibel carp with high bootstrap values (100% neighbor-joining, NJ; 100% maximum parsimony, MP) and M. rotundus from common bream composed a new cluster with high bootstrap values (100% NJ, 100% MP). From the morphological and molecular biological data, we gain enough evidences to support the validity of M. turpisrotundus.