Developmental role of PTHrP in murine molars
Developmental role of PTHrP in murine molars
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DOI：
10.1111/j.1600-0722.1998.tb02167.x
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发表时间：
1998-01-01
影响因子：
1.9
通讯作者：
Kurisu, K
中科院分区：
文献类型：
--
作者：
Liu, JG;Tabata, MJ;Kurisu, K
虽然PTHrP在多种胎儿组织中都有表达，但其确切的生理功能仍不清楚。本研究旨在阐明PTHrP在牙齿发育中的生物学作用。在牙胚中，PTHrP及其受体基因分别在釉质器官和牙间充质中表达，当小鼠牙齿外植体在无血清介质中与针对小鼠PTHrP mRNA的反义寡核苷酸(ODN)共同培养时，观察到牙胚骨组织的侵袭。另一方面，无ODN和有正义ODN培养的外植体显示出与体内观察到的正常组织结构相似的组织结构。这些结果表明，PTHrP在牙齿发育和保护牙胚免受骨组织侵袭方面是必不可少的。
Although PTHrP is produced in multiple fetal tissues, its precise physiological functions have yet to be clearly elucidated. The present study was undertaken to elucidate the biological role of PTHrP in the development of tooth. In I at tooth germs, the PTHrP and its receptor genes were expressed in the enamel organ and dental mesenchyme, respectively, When mouse tooth explants were cultured with antisense oligodeoxyribonucleotides (ODN) against mouse PTHrP mRNA in serum-free medium, an invasion of bone tissue was observed in the tooth germs. On the other hand, the explants cultured without ODN or with sense ODN showed normal histological structures similar to those observed in vivo. These results suggest that PTHrP is essential for tooth development and for the protection of tooth germs from the invasion of bone tissue.