Complete genome sequence of the dehalorespiring bacterium Desulfitobacterium hafniense Y51 and comparison with Dehalococcoides ethenogenes 195
Complete genome sequence of the dehalorespiring bacterium Desulfitobacterium hafniense Y51 and comparison with Dehalococcoides ethenogenes 195
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DOI：
10.1128/jb.188.6.2262-2274.2006
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发表时间：
2006-03-01
影响因子：
3.2
通讯作者：
Yukawa, H
中科院分区：
文献类型：
--
作者：
Nonaka, H;Keresztes, G;Yukawa, H
脱硫杆菌菌株具有在厌氧条件下通过脱卤呼吸使卤代化合物脱氯的能力。四氯乙烯脱氯菌Desulfitobacterium Bronniense Y51的完整基因组是一个5，727，534-bp的环状染色体，包含5，060个预测的蛋白质编码序列。该基因组仅包含两个还原脱卤酶基因，比大多数其他dehalorespiring菌株中报道的数量要少。二甲亚砜还原酶超家族的50多个成员和富马酸还原酶的黄素蛋白亚基的30个旁系同源物也被编码。基因组的一个显着特征是大量的O-脱甲基酶旁系同源物，其允许利用木质素衍生的苯基甲基醚作为电子供体。大的基因组揭示了一种更通用的微生物，它可以利用比以前认为的更大的一组专门的电子供体和受体。这与PCE脱氯菌株Dehalocococcoides ethenogenes 195形成鲜明对比，后者具有相对较小的基因组和狭窄的代谢谱。对这两种非常不同的菌株进行基因组比较，使我们能够缩小脱氯过程中涉及的潜在候选人。我们的研究结果提供了进一步的动力，利用嗜热菌作为生物修复的工具。
Desulfitobacterium strains have the ability to dechlorinate halogenated compounds under anaerobic conditions by dehalorespiration. The complete genome of the tetrachloroethene (PCE)-dechlorinating strain Desulfitobacterium hafniense Y51 is a 5,727,534-bp circular chromosome harboring 5,060 predicted protein coding sequences. This genome contains only two reductive dehalogenase genes, a lower number than reported in most other dehalorespiring strains. More than 50 members of the dimethyl sulfoxide reductase superfamily and 30 paralogs of the flavoprotein subunit of the fumarate reductase are encoded as well. A remarkable feature of the genome is the large number of O-demethylase paralogs, which allow utilization of lignin-derived phenyl methyl ethers as electron donors. The large genome reveals a more versatile microorganism that can utilize a larger set of specialized electron donors and acceptors than previously thought. This is in sharp contrast to the PCE-dechlorinating strain Dehalococcoides ethenogenes 195, which has a relatively small genome with a narrow metabolic repertoire. A genomic comparison of these two very different strains allowed us to narrow down the potential candidates implicated in the dechlorination process. Our results provide further impetus to the use of desulfitobacteria as tools for bioremediation.