ISOLATION AND CHARACTERIZATION OF A MOUSE INTERLEUKIN CDNA CLONE THAT EXPRESSES B-CELL STIMULATORY FACTOR-I ACTIVITIES AND T-CELL-STIMULATING AND MAST-CELL-STIMULATING ACTIVITIES
ISOLATION AND CHARACTERIZATION OF A MOUSE INTERLEUKIN CDNA CLONE THAT EXPRESSES B-CELL STIMULATORY FACTOR-I ACTIVITIES AND T-CELL-STIMULATING AND MAST-CELL-STIMULATING ACTIVITIES
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DOI：
10.1073/pnas.83.7.2061
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发表时间：
1986-04-01
影响因子：
11.1
通讯作者：
ARAI, K
中科院分区：
文献类型：
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作者：
LEE, F;YOKOTA, T;ARAI, K
从一个小鼠辅助性T细胞cDNA文库中分离出了一种编码独特小鼠白细胞介素的cDNA序列，该白细胞介素具有刺激B细胞、T细胞和肥大细胞的活性。这个在pcD表达载体中构建的文库，通过用DNA池转染COS猴细胞以表达由全长cDNA插入片段编码的产物来进行筛选。通过检测转染细胞的上清液，我们鉴定出了编码一种刺激T细胞和肥大细胞系的因子的克隆。这种因子还能诱导静息B细胞Ia抗原的表达，并增强B细胞产生IgG1和IgE，这是β细胞刺激因子1的两个特性。该DNA序列编码一个含140个氨基酸残基的多肽，其中包括一个假定的信号肽。这些结果表明，一个不同于白细胞介素2和白细胞介素3的单一cDNA克隆编码一种具有多种生物学活性的多肽。
A cDNA sequence coding for a unique mouse interleukin that expresses B-cell-, T-cell, and mast-cell-stimulating activities has been isolated from a mouse helper T-cell cDNA library. The library, constructed in the pcD expression vector, was screened by transfecting COS monkey cells with DNA pools to express the products encoded by full-length cDNA inserts. By assaying the transfected cell supernatants, we identified clones encoding a factor that stimulates T-cell and mast cell lines. This factor also induces Ia expression of resting B cells and enhances IgG1 and IgE production by B cells, two properties of .beta.-cell-stimulatory factor 1. The DNA sequence codes for a polypeptide of 140 amino acid residues including a putative signal peptide. These results demonstrate that a single cDNA clone distinct from interleukin 2 and interleukin 3 encodes a polypeptide with multiple biological activities.