TRANSCRIPTIONAL AND POSTTRANSCRIPTIONAL CONTROL OF IMMUNOGLOBULIN MESSENGER-RNA PRODUCTION DURING LYMPHOCYTE-B DEVELOPMENT
TRANSCRIPTIONAL AND POSTTRANSCRIPTIONAL CONTROL OF IMMUNOGLOBULIN MESSENGER-RNA PRODUCTION DURING LYMPHOCYTE-B DEVELOPMENT
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DOI：
10.1093/nar/14.13.5431
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发表时间：
1986-07-11
影响因子：
14.9
通讯作者：
PERRY, RP
中科院分区：
文献类型：
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作者：
KELLEY, DE;PERRY, RP
利用代表B淋巴细胞不同饱和阶段的多种细胞系，通过μ-δ位点分析转录模式的发育变化以及μ mRNA积累与转录活性之间的关系。正如早期研究所预期的那样，我们观察到，与携带表面IgM或表面IgM和IgD的细胞相比，IgM分泌细胞中μ m切割/poly A添加位点和δ1外显子之间区域的RNA聚合酶加载显著降低。在一些分泌IgM的杂交瘤中，转录终止主要发生在第一外显子的下游。因此，这些细胞中μ s终止的转录物的优势似乎主要由RNA加工事件决定，最有可能是通过μspoly A位点的更有效切割和/或Cμ和μ mexon的较低效率剪接。在两个浆细胞瘤细胞系中，μ和μ m位点之间的聚合酶卸载也对高μsmRNA表型有显著贡献。我们的结果进一步表明，转录后调节是IgM分泌细胞中μ mRNA大量积累的主要原因。有趣的是，不育的-μ RNA组分似乎不受这种转录后调控。
A variety of cell lines representing different saturation stages of the B lymphocyte were used to analyse developmental changes in the transcriptional pattern through the μ-δ locus and the relationship between μ mRNA accumulation and transcriptional activity. As anticipated from earlier studies, we observed that RNA polymerase loading in the region between the μmcleavage/poly A addition site and the δ1 exon is markedly decreased in IgM secreting cells compared to cells bearing surface IgM or surface IgM and IgD. In several IgM secreting hybridomas, transcriptional termination mainly occurred downstream of the first exon. Thus, the predominance of μs-terminated transcripts in these cells would appear to be principally determined by RNA processing events, most likely by more efficient cleavage at the μspoly A site and/or less efficient splicing of the Cμ and μmexons. In two plasmacytoma lines, polymerase unloading between the μsand μmsites also contributed significantly to the high μsmRNA phenotype. Our results further indicate that posttranscriptional regulation is largely responsible for the greatly increased accumulation of μ mRNA in the IgM secretors. Interestingly, the sterile-μ RNA components do not seem to be subject to this posttranscriptional regulation.