Genetic linkage map construction and QTL mapping of cadmium accumulation in radish (Raphanus sativus L.)
Genetic linkage map construction and QTL mapping of cadmium accumulation in radish (Raphanus sativus L.)
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DOI：
10.1007/s00122-012-1858-y
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发表时间：
2012-04
影响因子：
5.4
通讯作者：
Liang Xu;Liang-ju Wang;Y. Gong;W. Dai;Yan Wang;Xianwen Zhu;Tiancai Wen;Liwang Liu
中科院分区：
文献类型：
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作者：
Liang Xu;Liang-ju Wang;Y. Gong;W. Dai;Yan Wang;Xianwen Zhu;Tiancai Wen;Liwang Liu
镉（Cd）是一种广泛存在的土壤污染物，通过食物链对人类健康构成重大威胁。萝卜根和地上部镉含量存在较大的表型差异。然而，萝卜镉积累的遗传和分子机制仍有待阐明。本研究利用高镉积累品种NAU-Dysx和低镉积累品种NAU-Yh杂交的F2作图群体构建了一张遗传连锁图谱。该连锁图由523个SRAP、RAPD、SSR、ISSR、RAMP和RGA标记组成，总长度为1678.2cM，标记间平均距离为3.4cM。所有的标记分布在9个连锁群（LGs）具有134.7和236.8 cM之间的大小。4个影响根系镉积累的数量性状位点（QTL）定位在第1、4、6和9个LGS上，贡献率为9.86%~ 48.64%。在LG 1和LG 3上检测到2个与地上部Cd积累量相关的QTL，分别解释了17.08%和29.53%的表型变异。在LG 9上检测到一个主效QTL qRCd 9（LG 9根Cd积累量QTL），该QTL位于NAUrp 011_754和EM 5me6_286标记之间，LOD值为23.6，可解释F2植株Cd积累量表型总方差的48.64%。结果表明，qRCd 9是一个新的控制萝卜根系Cd积累的QTL，与该QTL紧密连锁的特异分子标记可用于低Cd萝卜育种的分子标记辅助选择。
Cadmium (Cd) is a widespread soil pollutant and poses a significant threat to human health via the food chain. Large phenotypic variations in Cd concentration of radish roots and shoots have been observed. However, the genetic and molecular mechanisms of Cd accumulation in radish remain to be elucidated. In this study, a genetic linkage map was constructed using an F2mapping population derived from a cross between a high Cd-accumulating cultivar NAU-Dysx and a low Cd-accumulating cultivar NAU-Yh. The linkage map consisted of 523 SRAP, RAPD, SSR, ISSR, RAMP, and RGA markers and had a total length of 1,678.2 cM with a mean distance of 3.4 cM between two markers. All mapped markers distributed on nine linkage groups (LGs) having sizes between 134.7 and 236.8 cM. Four quantitative trait loci (QTLs) for root Cd accumulation were mapped on LGs 1, 4, 6, and 9, which accounted for 9.86 to 48.64 % of all phenotypic variance. Two QTLs associated with shoot Cd accumulation were detected on LG1 and 3, which accounted for 17.08 and 29.53 % of phenotypic variance, respectively. A major-effect QTL,qRCd9(QTL for root Cd accumulation on LG9), was identified on LG 9 flanked by NAUrp011_754 and EM5me6_286 markers with a high LOD value of 23.6, which accounted for 48.64 % of the total phenotypic variance in Cd accumulation of F2lines. The results indicated thatqRCd9is a novel QTL responsible for controlling root Cd accumulation in radish, and the identification of specific molecular markers tightly linked to the major QTL could be further applied for marker-assisted selection (MAS) in low-Cd content radish breeding program.