Microstructural and histochemical variations of Korshinsk peashrub during aqueous ammonia pretreatment and its impacts on enzymatic digestibility of cellulose
Microstructural and histochemical variations of Korshinsk peashrub during aqueous ammonia pretreatment and its impacts on enzymatic digestibility of cellulose
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氨水预处理过程中科尔申斯克豌豆的微观结构和组织化学变化及其对纤维素酶消化率的影响
DOI：
10.1016/j.jece.2022.108613
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发表时间：
2022-09
影响因子：
7.7
通讯作者：
Guanhua Li
中科院分区：
文献类型：
--
作者：
Yin Liu;Huiting Xue;Caiyun Miao;Guanhua Li
科尔辛斯克灌木（KP）是一种耐旱的多年生能量灌木。KP的增值有利于环境保护和经济发展。本文首次采用水氨（AA）预处理来增强KP的酶解，并通过湿化学组成、物理性质和糖谱分析来评估AA预处理对KP顽固性的影响。结果表明，80◦C AA预处理可降解9.85%以上的中性洗涤剂，保留51.63%的纤维素。AA预处理可以明显消除非纤维素聚合物，如果胶/阿拉伯半乳聚糖表位，并中断酯键和果胶连接基质，导致整个细胞壁完整性的破坏。预处理KP（80◦C）的还原糖产率最高，为316.93 mg/g，是未处理KP的1.96倍。常温AA预处理可能是生物炼制KP酶解糖化的一种很有前途的方法。
Korshinsk peashrub (KP) is a drought-tolerant perennial energy shrub. Valorization of KP is beneficial to both environment protection and economy development. Herein, aqueous ammonia (AA) pretreatment was applied to enhance enzymatic hydrolysis of KP for the first time, and influences of AA pretreatment on recalcitrance of KP were also assessed through wet chemistry composition, physical properties, and glycome profiling analysis. The results suggested that AA pretreatment at 80◦C degraded over 9.85% neutral detergent soluble and retained 51.63% cellulose. AA pretreatment could explicitly eliminate non-cellulosic polymers, e.g. pectin/arabinogalactan epitopes, and interrupt ester bond and pectin linking matrix, leading to deconstruction in overall cell wall integrity. The pretreated KP (80◦C) gave the highest reducing sugar yield of 316.93 mg/g, which was 1.96 times as high as the untreated KP. AA pretreatment at atmospheric temperature might be a promising strategy employed in enzymatic saccharification of KP in biorefinery.
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