Chemical variation in cell wall of sugar beet pulp caused by aqueous ammonia pretreatment influence enzymatic digestibility of cellulose
Chemical variation in cell wall of sugar beet pulp caused by aqueous ammonia pretreatment influence enzymatic digestibility of cellulose
复制标题
氨水预处理引起的甜菜浆细胞壁化学变化影响纤维素的酶消化率
DOI：
10.1016/j.indcrop.2020.112786
复制
发表时间：
2020-11-01
影响因子：
5.9
通讯作者：
Yuan, Lin
中科院分区：
文献类型：
--
作者：
Li, Guanhua;Zhang, Yue;Yuan, Lin
甜菜浆被认为是生产燃料乙醇的重要原料。本研究的目的是通过成像技术结合酶解动力学分析，探讨氨水处理引起的细胞壁化学变化与纤维素酶消化率的关系。结果表明，部分减少和重新分布的木质素会削弱木质素与纤维素之间的相互作用，使细胞壁上的羟基暴露出来，导致纤维素酶在纤维素膜上的吸附增加。在80℃条件下，SBP对纤维素酶的最大吸附量为370.37 fPU/g，比对照提高了37%。此外，还原糖产量、初始水解率和最大催化效率分别比对照提高121%、202%和125%。氨水通过削弱木质素和纤维素之间的相互作用，为提高纤维素的酶消化率提供了一条很好的途径，这可能是SBP生产燃料乙醇的一种合适的前处理方法。
Sugar beet pulp (SBP) is considered to be an important feedstock for the fuel ethanol production. The aim of this study is to investigate the relationship between chemical variation in cell wall caused by aqueous ammonia pretreatment and the enzymatic digestibility of cellulose through imaging techniques coupled with enzymatic hydrolysis kinetics analysis. The results revealed that the partly decreased and redistributed lignin could weaken interactions between lignin and cellulose, expose more hydroxyl groups on cell wall, and lead to more absorbed cellulase on cellulose. The maximum amount of adsorbed cellulase (370.37 FPU/g) was obtained from SBP (at 80 degrees C), which was 37 % more than that of the control. Additionally, the reducing sugar yield, initial hydrolysis rate, and maximum catalytic efficiency were 121 %, 202 %, and 125 % more than that of the control, respectively. Aqueous ammonia provided a good approach for improving enzymatic digestibility of cellulose through weakening the interactions between lignin and cellulose, which might be a suitable pretreatment in fuel ethanol production from SBP.