SPECIFIC EXPRESSION OF N-ACETYLASPARTATE IN NEURONS, OLIGODENDROCYTE-TYPE-2 ASTROCYTE PROGENITORS, AND IMMATURE OLIGODENDROCYTES INVITRO
SPECIFIC EXPRESSION OF N-ACETYLASPARTATE IN NEURONS, OLIGODENDROCYTE-TYPE-2 ASTROCYTE PROGENITORS, AND IMMATURE OLIGODENDROCYTES INVITRO
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DOI：
10.1111/j.1471-4159.1992.tb08875.x
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发表时间：
1992-07-01
影响因子：
4.7
通讯作者：
NOBLE, M
中科院分区：
文献类型：
--
作者：
URENJAK, J;WILLIAMS, SR;NOBLE, M
为了测试N-乙酰天冬氨酸（NAA）作为质子核磁共振（H-1 NMR）光谱的神经元标记物的特异性，使用H-1 NMR和HPLC分析纯化和表征的培养细胞的NAA含量。研究的细胞类型包括小脑颗粒神经元、1型星形胶质细胞、脑膜细胞、少突胶质细胞2型星形胶质细胞（O-2A）祖细胞和少突胶质细胞。高浓度的NAA被发现在小脑颗粒神经元的提取物（约12 nmol/mg的蛋白质），而NAA仍然无法检测到纯化的1型星形胶质细胞，脑膜细胞，和成熟的少突胶质细胞。然而，在体外生长的O-2A祖细胞中发现了两倍的神经元水平的NAA。此外，在未成熟的少突胶质细胞培养物中也检测到显着水平的NAA。我们的数据部分支持以前的建议，NAA可能是一个有用的神经元标记物的H-1 NMR光谱检查成人大脑。然而，他们也提出了进一步的可能性，即NAA的改变与一些特定的大脑疾病，特别是在新生儿和幼儿中观察到的疾病，可能反映了少突胶质细胞或其前体细胞发育的异常。
To test the specificity of N-acetylaspartate (NAA) as a neuronal marker for proton nuclear magnetic resonance ( H-1 NMR) spectroscopy, purified and characterized cultured cells were analyzed for their NAA content using both H-1 NMR and HPLC. Cell types studied included cerebellar granule neurons, type-1 astrocytes, meningeal cells, oligodendrocyte-type-2 astrocyte (O-2A) progenitor cells, and oligodendrocytes. A high concentration of NAA was found in extracts of cerebellar granule neurons (approximately 12 nmol/mg of protein), whereas NAA remained undetectable in purified type-1 astrocytes, meningeal cells, and mature oligodendrocytes. However, twice the neuronal level of NAA was found in O-2A progenitors grown in vitro. In addition significant levels of NAA were also detected in cultures of immature oligodendrocytes. Our data partly support previous suggestions that NAA may be a useful neuronal marker for H-1 NMR spectroscopic examination of the adult brain. However, they also raise the further possibility that alterations of NAA associated with some specific brain disorders, particularly disorders seen in newborn and young children, may reflect abnormalities in the development of oligodendroglia or their precursors.