Protective effect of berberine on antioxidant enzymes and positive transcription elongation factor b expression in diabetic rat liver
Protective effect of berberine on antioxidant enzymes and positive transcription elongation factor b expression in diabetic rat liver
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DOI：
10.1016/j.fitote.2010.08.019
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发表时间：
2011-03-01
期刊：
影响因子：
3.4
通讯作者：
Zhou, Shi-Wen
中科院分区：
文献类型：
--
作者：
Zhou, Ji-Yin;Zhou, Shi-Wen
观察小檗碱对2型糖尿病大鼠血清和肝组织抗氧化、抗脂质过氧化及肝组织正转录延伸因子B b（P-TEF B b）表达的影响。过夜禁食大鼠腹腔注射35 mg/kg链脲佐菌素。糖尿病大鼠在2周后入院，并给予高碳水化合物/高脂肪饮食以诱导高血压。从第16周开始，分别给予75、150、300 mg/kg黄连素、100 mg/kg非诺贝特或4 mg/kg罗格列酮治疗16周。分别采用真实的时间PCR和免疫组化方法检测肝组织中P-TEFb（由细胞周期蛋白依赖性激酶9和细胞周期蛋白T1组成）mRNA和蛋白的表达。黄连素显著上调糖尿病大鼠肝脏细胞周期蛋白依赖性激酶9、细胞周期蛋白T1 mRNA和蛋白的表达。黄连素可明显降低糖尿病大鼠肝组织和血清中丙二醛水平，升高肝组织和血清中过氧化氢酶、超氧化物歧化酶、谷胱甘肽过氧化物酶和谷胱甘肽的活性。这些结果表明，小檗碱上调P-TEFb表达，抗氧化和抗脂质过氧化作用可能与其对糖尿病的保护作用有关。(C)2010 Elsevier B. V.保留所有权利。
The protective effect of berberine against antioxidant, antilipid peroxidation in serum and liver tissue, and positive transcription elongation factor b (P-TEFb) expression in liver tissue of type 2 diabetic rats was investigated. Overnight fasted rats were intraperitoneally injected 35 mg/kg streptozotocin. Diabetic rats were admitted after 2 weeks and given a high-carbohydrate/high-fat diet to induce hyperlipidemias. From week 16, diabetic rats were treated with 75, 150, 300 mg/kg berberine, 100 mg/kg fenofibrate or 4 mg/kg rosiglitazone for another 16 weeks. P-TEFb (composed of cyclin-dependent kinase 9 and cyclin T1) mRNA and protein expression in liver tissue were detected by real time PCR and immunohistochemistry, respectively. Berberine significantly up-regulated the declined cyclin-dependent kinase 9, cyclin T1 mRNA and protein expression in diabetic rat liver. Berberine obviously decreased malondialdehyde level and increased catalase, superoxide dismutase, glutathione peroxidase, and glutathione activities in liver tissue and serum of diabetic rats. These results suggest that the effects of berberine on up-regulation of P-TEFb expression, antioxidant and antilipid peroxidation may be related to its protective potential on diabetes. (C) 2010 Elsevier B.V. All rights reserved.