Immunohistochemical description of nitric oxide synthase isoforms in human clitoris
Immunohistochemical description of nitric oxide synthase isoforms in human clitoris
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DOI：
10.1097/00005392-199707000-00020
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发表时间：
1997-07-01
影响因子：
6.6
通讯作者：
Docimo, SG
中科院分区：
文献类型：
--
作者：
Burnett, AL;Calvin, DC;Docimo, SG
目的：我们的目的是鉴定和定位一氧化氮合酶亚型在人类阴蒂中，以支持一氧化氮介导该器官勃起功能的假设。材料和方法：一氧化氮合酶免疫组织化学研究特异性神经元，在从4名患者获得的人阴蒂组织上进行酶的诱导型和内皮型（3例为女性假两性畸形，1例为真两性畸形）和1例尸检时表型正常的女性。在阴蒂头和阴蒂海绵体内的神经束和纤维中检测到神经元型一氧化氮合酶免疫反应阳性，在后一种组织中占主导地位。未发现特异性诱导型一氧化氮合酶免疫反应性。内皮型一氧化氮合酶免疫反应检测血管和窦内皮细胞的这些组织中的优势在阴蒂头。结论：一氧化氮合酶亚型在人类阴蒂的存在和解剖定位表明，一氧化氮是在这个器官中产生。这些数据表明，一氧化氮可能作为阴蒂平滑肌活动的调节剂参与阴蒂的勃起生理。需要功能研究来支持这一假设。
Purpose: Our aim was to identify and localize nitric oxide synthase isoforms in the human clitoris in support of the hypothesis that nitric oxide mediates erectile function in this organ.Materials and Methods: Nitric oxide synthase immunohistochemistry studies specific for neuronal, inducible and endothelial isoforms of the enzyme were performed on human clitoral tissue obtained from 4 patients (3 with female pseudohermaphroditism and 1 with true hermaphroditism) at feminizing genitoplasty and from I phenotypically normal woman at autopsy.Results: Neuronal nitric oxide synthase immunoreactivity was detected in nerve bundles and fibers coursing within the glans clitoris and corpora cavernosa of the clitoris, predominating in the latter tissue. Specific inducible nitric oxide synthase immunoreactivity was not identified. Endothelial nitric oxide synthase immunoreactivity was detected in vascular and sinusoidal endothelium of these tissues with a predominance in the glans clitoris.Conclusions: The presence and anatomical localizations of nitric oxide synthase isoforms in the human clitoris indicate that nitric oxide is generated in this organ. These data suggest that nitric oxide may be involved in the erectile physiology of the clitoris as a modulator of clitoral smooth muscle activity. Functional studies are required to support this hypothesis.