LUSH odorant-binding protein mediates chemosensory responses to alcohols in Drosophila melanogaster.
LUSH odorant-binding protein mediates chemosensory responses to alcohols in Drosophila melanogaster.
复制标题
LUSH 气味结合蛋白介导果蝇对酒精的化学感应反应。
DOI：
10.1093/genetics/150.2.711
复制
发表时间：
1998
期刊：
影响因子：
3.3
通讯作者：
Smith,DP
中科院分区：
文献类型：
--
作者：
Kim,MS;Repp,A;Smith,DP
介导昆虫化学感觉辨别的分子机制尚不清楚。使用增强子捕获的方法，我们确定了一个新的果蝇突变体，郁郁葱葱的，气味特异性嗅觉行为的缺陷。茂盛的突变果蝇异常地被高浓度的乙醇、丙醇和丁醇吸引，但对其他气味剂具有正常的化学感受反应。我们表明，野生型苍蝇有一个积极的嗅觉回避机制，以防止吸引到浓酒精，这种反应是有缺陷的郁郁葱葱的突变体。这表明与茂盛突变相关的嗅觉行为缺陷可能是由化学回避的特定缺陷引起的。郁郁葱葱的突变体有一个3-kb的缺失，产生无脊椎动物气味结合蛋白家族新成员LUSH的无效等位基因。LUSH通常仅在位于第三触角节腹侧表面的一个亚组的类昆虫化学感受器中表达。LUSH由非神经元支持细胞分泌到感受器淋巴中，感受器淋巴使这些感受器内的嗅觉神经元沐浴。重新引入一个克隆的野生型拷贝的郁郁葱葱的突变体的背景完全恢复野生型嗅觉行为，表明这种气味结合蛋白是需要在一个子集的感受器正常的化学感觉行为的一个子集的气味。这些研究结果提供了直接的证据，气味结合蛋白是果蝇正常化学感觉行为所必需的，并可能部分决定嗅觉神经元在体内的化学特异性。
The molecular mechanisms mediating chemosensory discrimination in insects are unknown. Using the enhancer trapping approach, we identified a new Drosophila mutant, lush, with odorant-specific defects in olfactory behavior. lush mutant flies are abnormally attracted to high concentrations of ethanol, propanol, and butanol but have normal chemosensory responses to other odorants. We show that wild-type flies have an active olfactory avoidance mechanism to prevent attraction to concentrated alcohol, and this response is defective in lush mutants. This suggests that the defective olfactory behavior associated with the lush mutation may result from a specific defect in chemoavoidance. lush mutants have a 3-kb deletion that produces a null allele of a new member of the invertebrate odorant-binding protein family, LUSH. LUSH is normally expressed exclusively in a subset of trichoid chemosensory sensilla located on the ventral-lateral surface of the third antennal segment. LUSH is secreted from nonneuronal support cells into the sensillum lymph that bathes the olfactory neurons within these sensilla. Reintroduction of a cloned wild-type copy of lush into the mutant background completely restores wild-type olfactory behavior, demonstrating that this odorant-binding protein is required in a subset of sensilla for normal chemosensory behavior to a subset of odorants. These findings provide direct evidence that odorant-binding proteins are required for normal chemosensory behavior in Drosophila and may partially determine the chemical specificity of olfactory neurons in vivo.