A MONOCLONAL-ANTIBODY TO THE HUMAN C-ERBB3 PROTEIN STIMULATES THE ANCHORAGE-INDEPENDENT GROWTH OF BREAST-CANCER CELL-LINES
A MONOCLONAL-ANTIBODY TO THE HUMAN C-ERBB3 PROTEIN STIMULATES THE ANCHORAGE-INDEPENDENT GROWTH OF BREAST-CANCER CELL-LINES
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DOI：
10.1038/bjc.1994.328
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发表时间：
1994-09-01
影响因子：
8.8
通讯作者：
GULLICK, WJ
中科院分区：
文献类型：
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作者：
RAJKUMAR, T;GULLICK, WJ
c-erbB 3蛋白是I型生长因子受体家族的成员。它在正常组织中具有广泛的表达模式，并且在约20%的乳腺癌中过表达。我们已经提出了一种特异性单克隆抗体，称为SGP 1，针对c-erbB 3的细胞外结构域，其识别该蛋白的天然形式。发现该单克隆抗体适度但显著地刺激乳腺肿瘤细胞系BT483和T47 D的锚定非依赖性克隆效率，这两种细胞系均表达c-erbB 3蛋白。在缺乏表达的293细胞上没有观察到效果，对照同种型匹配的抗体也没有促进任何测试的鳗鱼的生长。这些结果表明c-erbB 3蛋白通常可能充当生长因子受体。
The c-erbB3 protein is a member of the type I growth factor receptor family. It has a widespread pattern of expression in normal tissues and is overexpressed in about 20% of breast cancers. We have raised a specific monoclonal antibody, called SGP1, against the extracellular domain of c-erbB3 which recognises the native form of the protein. The monoclonal antibody was found to modestly but significantly stimulate the anchorage-independent cloning efficiency of the breast tumour cell lines BT483 and T47D, both of which express the c-erbB3 protein. No effect was observed on 293 cells lacking expression, nor did a control isotype-matched antibody promote the growth of any of the eels tested. These results suggest that the c-erbB3 protein may normally act as a growth factor receptor.