A turn-on coordination nanoparticle-based fluorescent probe for phosphate in human serum.
A turn-on coordination nanoparticle-based fluorescent probe for phosphate in human serum.
复制标题
DOI：
10.1039/c5nr00515a
复制
发表时间：
2015-03
期刊：
影响因子：
6.7
通讯作者：
Na Lin;Jian Li;Zhixiang Lu;Longchun Bian;Liyan Zheng;Qiue Cao;Z. Ding
中科院分区：
文献类型：
--
作者：
Na Lin;Jian Li;Zhixiang Lu;Longchun Bian;Liyan Zheng;Qiue Cao;Z. Ding
配位纳米粒子（CNPs）由于其独特的可调特性为设计各种发光纳米探针提供了机会，正成为化学传感应用的有吸引力的平台。在这里，我们提出了一种基于cnp的荧光纳米探针，其中荧光团（罗丹明B， RB）和猝灭剂（亚甲基蓝，MB）被腺嘌呤、联苯-4,4'-二羧酸（BDA）和锌离子自组装的配位网络自发包裹。CNPs中荧光团和猝灭团的聚集导致RB的猝灭态荧光。在磷酸的存在下，RB和MB可以从CNPs中释放出来，从而触发RB的荧光。基于识别驱动拆卸原理，研制了一种具有宽响应范围（0.5 ~ 50 μM）的新型PO4（3-）荧光探针，成功应用于人血清样品中磷酸盐的检测。这项工作不仅开发了一种磷酸盐探针，而且为通过改变有机连接剂或金属离子来设计针对各种靶标的纳米探针或纳米载体提供了一般策略。
Coordination nanoparticles (CNPs) are becoming attractive platforms for chemical sensing applications because their unique adjustable properties offer the opportunity to design various luminescent nanoprobes. Here, we present a CNP-based fluorescent nanoprobe, in which fluorophores (rhodamine B, RB) and quenchers (methylene blue, MB) were spontaneously enfolded by coordination networks self-assembled of adenine, biphenyl-4,4'-dicarboxylic acid (BDA) and zinc ions. The aggregation of fluorophores and quenchers in CNPs resulted in a quenched state fluorescence of RB. RB and MB could be released from CNPs in the presence of phosphate, which triggered the fluorescence of RB. On the basis of recognition-driven disassembly principle, a novel turn-on fluorescent probe for the determination of PO4(3-) with a wide response range (0.5-50 μM) has been successfully applied in the detection of phosphate in human serum samples. This work not only develops a probe for phosphate but also provides a general strategy for designing nanoprobes or nanocarriers towards various targets by altering organic linkers or metal ions.