Synthesis of sulfur isotope-labeled sulfate donor, 3'-phosphoadenosine-5'-phosphosulfate, for studying glycosaminoglycan functions.
Synthesis of sulfur isotope-labeled sulfate donor, 3'-phosphoadenosine-5'-phosphosulfate, for studying glycosaminoglycan functions.
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合成硫同位素标记的硫酸盐供体 3-磷酸腺苷-5-磷酸硫酸盐，用于研究糖胺聚糖功能。
DOI：
10.1007/978-1-4939-1714-3_5
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Kuberan,Balagurunathan
中科院分区：
文献类型：
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作者：
Mencio,Caitlin;Swarup,VimalP;Soliai,Marcus;Kuberan,Balagurunathan
The biological activity of glycosaminoglycans (GAGs) depends greatly on the sulfation pattern present within the GAG chain. Chemical biology of GAGs can be further advanced by preparation of sulfur-isotope-enriched sulfated GAGs. 3′-Phosphoadenosine-5′-phosphosulfate (PAPS) serves as a universal sulfate donor in the sulfation of GAGs by sulfotransferases. Therefore, synthesis of PAPS carrying sulfur isotopes is critical in the preparation of labeled GAGs for biochemical studies. Here we describe a robust in vitro enzymatic synthesis of sulfur isotope-enriched PAPS which allows for heavy- or radio-isotope labeling of GAG chains.
